(Circular) (Six-base) MAPSORT of: pFN21AA0148.seq Check: 9035 from: 1 to: 6748 pFN21AA0148 6748 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 3884 4558 278 Size: 53 83 186 936 1837 511 674 2468 Fragments arranged by size: 2468 1837 936 674 511 186 83 53 AccI GT'mk_AC Cuts at: 1937 4091 1937 Size: 2154 4594 Acc65I G'GTAC_C Cuts at: 3637 4069 3637 Size: 432 6316 AclI AA'CG_TT Cuts at: 1563 2087 4047 4875 5248 1563 Size: 524 1960 828 373 3063 Fragments arranged by size: 3063 1960 828 524 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 4811 6195 6463 1632 Size: 539 2640 1384 268 1917 Fragments arranged by size: 2640 1917 1384 539 268 AflII C'TTAA_G Cuts at: 820 1017 3742 820 Size: 197 2725 3826 Fragments arranged by size: 3826 2725 197 AflIII A'CryG_T Cuts at: 1203 3799 5647 6624 1203 Size: 2596 1848 977 1327 Fragments arranged by size: 2596 1848 1327 977 AgeI A'CCGG_T Cuts at: 5579 5579 Size: 6748 AhdI GACnn_n'nnGTC Cuts at: 2254 5477 2254 Size: 3223 3525 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6748 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6748 AlwNI CAG_nnn'CTG Cuts at: 4491 6063 4491 Size: 1572 5176 ApaLI G'TGCA_C Cuts at: 4805 5961 4805 Size: 1156 5592 ApoI r'AATT_y Cuts at: 1493 2318 3274 4057 4283 4435 6544 1493 Size: 825 956 783 226 152 2109 1697 Fragments arranged by size: 2109 1697 956 825 783 226 152 AseI AT'TA_AT Cuts at: 160 1032 5302 160 Size: 872 4270 1606 Fragments arranged by size: 4270 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 AvaI C'yCGr_G Cuts at: 1870 1943 3097 3381 4073 1870 Size: 73 1154 284 692 4545 Fragments arranged by size: 4545 1154 692 284 73 BamHI G'GATC_C Cuts at: 2056 4078 4445 2056 Size: 2022 367 4359 Fragments arranged by size: 4359 2022 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2130 2637 3637 4069 5524 Size: 325 217 627 343 507 1000 432 1455 Cuts at: 5524 618 Size: 1842 Fragments arranged by size: 1842 1455 1000 627 507 432 343 325 217 BanII G_rGCy'C Cuts at: 1391 3123 3741 4067 1391 Size: 1732 618 326 4072 Fragments arranged by size: 4072 1732 618 326 BbeI G_GCGC'C Cuts at: 2134 2134 Size: 6748 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2278 3213 3713 3923 928 Size: 971 379 935 500 210 3753 Fragments arranged by size: 3753 971 935 500 379 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4939 4973 1903 Size: 34 3002 34 3678 Fragments arranged by size: 3678 3002 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3789 4640 5850 3789 Size: 851 1210 4687 Fragments arranged by size: 4687 1210 851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6748 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5359 136 Size: 107 122 71 631 605 3687 1525 Fragments arranged by size: 3687 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2398 6743 2398 Size: 4345 2403 BlpI GC'TnA_GC Cuts at: 2774 4165 2774 Size: 1391 5357 Bme1580I G_kGCm'C Cuts at: 1790 2640 4809 5965 1790 Size: 850 2169 1156 2573 Fragments arranged by size: 2573 2169 1156 850 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6748 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4479 5437 442 Size: 416 53 83 399 24 3062 958 1753 Fragments arranged by size: 3062 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6748 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3248 3467 3533 5408 1239 Size: 2009 219 66 1875 2579 Fragments arranged by size: 2579 2009 1875 219 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4873 5738 6036 4873 Size: 865 298 5585 Fragments arranged by size: 5585 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2192 3404 5411 882 Size: 617 693 1212 2007 2219 Fragments arranged by size: 2219 2007 1212 693 617 BsaAI yAC'GTr Cuts at: 493 1204 3802 4539 493 Size: 711 2598 737 2702 Fragments arranged by size: 2702 2598 737 711 BsaBI GATnn'nnATC Cuts at: 6742 6742 Size: 6748 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2131 3066 3370 3881 Size: 53 83 186 936 598 935 304 511 Cuts at: 3881 4555 4937 5572 275 Size: 674 382 635 1451 Fragments arranged by size: 1451 936 935 674 635 598 511 382 304 186 83 53 BsaWI w'CCGG_w Cuts at: 2019 2962 3413 5181 5579 5853 6000 6411 2019 Size: 943 451 1768 398 274 147 411 2356 Fragments arranged by size: 2356 1768 943 451 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3420 3450 1178 Size: 30 165 30 2017 30 4476 Fragments arranged by size: 4476 2017 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3088 1186 Size: 1902 4846 BseYI C'CCAG_C Cuts at: 5951 6447 5951 Size: 496 6252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6175 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2949 4198 4959 5987 1540 Size: 275 186 948 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 948 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2476 3123 3741 4809 4894 5965 2476 Size: 647 618 1068 85 1071 3259 Fragments arranged by size: 3259 1071 1068 647 618 85 BsiWI C'GTAC_G Cuts at: 3987 3987 Size: 6748 BsmI GAATG_Cn' Cuts at: 2236 4259 4352 2236 Size: 2023 93 4632 Fragments arranged by size: 4632 2023 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2882 3361 2882 Size: 479 6269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2476 2640 3123 3741 4067 4809 4894 Size: 399 686 164 483 618 326 742 85 Cuts at: 4894 5965 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 686 618 483 399 326 164 85 BspEI T'CCGG_A Cuts at: 2019 6411 2019 Size: 4392 2356 BspHI T'CATG_A Cuts at: 4637 6367 4637 Size: 1730 5018 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3961 4103 844 Size: 3117 142 3489 Fragments arranged by size: 3489 3117 142 BsrBI CCG'CTC Cuts at: 1394 1823 4635 1394 Size: 429 2812 3507 Fragments arranged by size: 3507 2812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5243 5417 65 Size: 1183 3995 174 1396 Fragments arranged by size: 3995 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3243 5392 5579 1700 Size: 110 1433 2149 187 2869 Fragments arranged by size: 2869 2149 1433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6748 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6748 BssSI C'ACGA_G Cuts at: 2660 4808 5820 2660 Size: 2148 1012 3588 Fragments arranged by size: 3588 2148 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 2804 794 Size: 1448 562 4738 Fragments arranged by size: 4738 1448 562 BstBI TT'CG_AA Cuts at: 3278 5552 3278 Size: 2274 4474 BstEII G'GTnAC_C Cuts at: 1184 2932 4018 1184 Size: 1748 1086 3914 Fragments arranged by size: 3914 1748 1086 BstXI CCAn_nnnn'nTGG Cuts at: 3565 4015 3565 Size: 450 6298 BstYI r'GATC_y Cuts at: 1972 2056 2398 4078 4445 4830 4847 6299 6385 Size: 84 342 1680 367 385 17 1452 86 Cuts at: 6385 6397 6743 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1680 1452 385 367 346 342 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 3284 3284 Size: 6748 BtgI C'CryG_G Cuts at: 513 1065 2168 2856 513 Size: 552 1103 688 4405 Fragments arranged by size: 4405 1103 688 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2342 3979 487 Size: 683 56 1116 1637 3256 Fragments arranged by size: 3256 1637 1116 683 56 BtsI GCAGTG_nn' Cuts at: 2116 2180 3225 4264 5058 5078 2116 Size: 64 1045 1039 794 20 3786 Fragments arranged by size: 3786 1045 1039 794 64 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6748 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6713 DraI TTT'AAA Cuts at: 4052 4052 Size: 6748 DraIII CAC_nnn'GTG Cuts at: 2169 2169 Size: 6748 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5755 809 Size: 507 4439 1802 Fragments arranged by size: 4439 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3620 4195 5084 Size: 54 1425 50 275 18 1790 575 889 Cuts at: 5084 6713 8 Size: 1629 43 Fragments arranged by size: 1790 1629 1425 889 575 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4195 1537 Size: 275 2383 4090 Fragments arranged by size: 4090 2383 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3361 3459 4678 1353 Size: 2008 98 1219 3423 Fragments arranged by size: 3423 2008 1219 98 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5335 5709 5855 387 Size: 1064 138 3746 374 146 1280 Fragments arranged by size: 3746 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3121 3739 3121 Size: 618 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 3248 3467 3533 4811 5408 6195 Size: 393 539 1077 219 66 1278 597 787 Cuts at: 6195 6463 1239 Size: 268 1524 Fragments arranged by size: 1524 1278 1077 787 597 539 393 268 219 66 EcoNI CCTnn'n_nnAGG Cuts at: 2499 2499 Size: 6748 EcoO109I rG'GnC_Cy Cuts at: 3931 3931 Size: 6748 EcoRI G'AATT_C Cuts at: 2318 4057 4435 2318 Size: 1739 378 4631 Fragments arranged by size: 4631 1739 378 FspI TGC'GCA Cuts at: 5254 5254 Size: 6748 HaeII r_GCGC'y Cuts at: 1678 2134 2659 2928 5895 1678 Size: 456 525 269 2967 2531 Fragments arranged by size: 2967 2531 525 456 269 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 2966 2998 3401 Size: 32 135 32 668 32 694 32 403 Cuts at: 3401 3433 3923 3955 5389 5421 5463 5495 1373 Size: 32 490 32 1434 32 42 32 2626 Fragments arranged by size: 2626 1434 694 668 490 403 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2878 4092 4338 669 Size: 988 281 940 1214 246 3079 Fragments arranged by size: 3079 1214 988 940 281 246 HindIII A'AGCT_T Cuts at: 748 3251 748 Size: 2503 4245 HpaI GTT'AAC Cuts at: 4338 4338 Size: 6748 KasI G'GCGC_C Cuts at: 2130 2130 Size: 6748 KpnI G_GTAC'C Cuts at: 3641 4073 3641 Size: 432 6316 MfeI C'AATT_G Cuts at: 4347 4347 Size: 6748 MluI A'CGCG_T Cuts at: 6624 6624 Size: 6748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2201 3372 5862 6046 1305 Size: 896 1171 2490 184 2007 Fragments arranged by size: 2490 2007 1171 896 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3622 10 Size: 54 1425 343 1790 3136 Fragments arranged by size: 3136 1790 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 2861 3401 4706 5065 5224 518 Size: 648 1564 131 540 1305 359 159 2042 Fragments arranged by size: 2042 1564 1305 648 540 359 159 131 MspA1I CmG'CkG Cuts at: 1606 2049 3958 4015 4164 4841 5989 6234 1606 Size: 443 1909 57 149 677 1148 245 2120 Fragments arranged by size: 2120 1909 1148 677 443 245 149 57 NarI GG'CG_CC Cuts at: 2131 2131 Size: 6748 NcoI C'CATG_G Cuts at: 513 1065 2856 513 Size: 552 1791 4405 Fragments arranged by size: 4405 1791 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6748 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6748 NotI GC'GGCC_GC Cuts at: 4195 4195 Size: 6748 NspI r_CATG'y Cuts at: 1132 1229 4106 5651 1132 Size: 97 2877 1545 2229 Fragments arranged by size: 2877 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3926 1348 Size: 2578 4170 PciI A'CATG_T Cuts at: 5647 5647 Size: 6748 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 3629 1108 Size: 1539 982 4227 Fragments arranged by size: 4227 1539 982 PmeI GTTT'AAAC Cuts at: 4052 4052 Size: 6748 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4817 4849 6330 6362 4817 Size: 32 1481 32 5203 Fragments arranged by size: 5203 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3931 3931 Size: 6748 PshAI GACnn'nnGTC Cuts at: 1613 2945 3373 3901 1613 Size: 1332 428 528 4460 Fragments arranged by size: 4460 1332 528 428 PsiI TTA'TAA Cuts at: 2747 4318 2747 Size: 1571 5177 PstI C_TGCA'G Cuts at: 830 2048 2111 3903 4100 830 Size: 1218 63 1792 197 3478 Fragments arranged by size: 3478 1792 1218 197 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6748 PvuII CAG'CTG Cuts at: 3958 4015 3958 Size: 57 6691 SacI G_AGCT'C Cuts at: 3123 3741 3123 Size: 618 6130 SalI G'TCGA_C Cuts at: 1936 4090 1936 Size: 2154 4594 SanDI GG'GwC_CC Cuts at: 3931 3931 Size: 6748 SbfI CC_TGCA'GG Cuts at: 4100 4100 Size: 6748 ScaI AGT'ACT Cuts at: 2978 4996 6708 2978 Size: 2018 1712 3018 Fragments arranged by size: 3018 2018 1712 SfcI C'TryA_G Cuts at: 826 1045 2044 2107 3899 4096 5231 5912 6103 Size: 219 999 63 1792 197 1135 681 191 Cuts at: 6103 826 Size: 1471 Fragments arranged by size: 1792 1471 1135 999 681 219 197 191 63 SfoI GGC'GCC Cuts at: 2132 2132 Size: 6748 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6748 SmaI CCC'GGG Cuts at: 1872 3383 4075 1872 Size: 1511 692 4545 Fragments arranged by size: 4545 1511 692 SmlI C'TyrA_G Cuts at: 820 1017 1943 3742 4852 5753 6015 820 Size: 197 926 1799 1110 901 262 1553 Fragments arranged by size: 1799 1553 1110 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4539 493 Size: 4046 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6748 SphI G_CATG'C Cuts at: 1132 4106 1132 Size: 2974 3774 SspI AAT'ATT Cuts at: 5 52 4672 5 Size: 47 4620 2081 Fragments arranged by size: 4620 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6748 StyI C'CwwG_G Cuts at: 513 1065 2856 3999 4187 513 Size: 552 1791 1143 188 3074 Fragments arranged by size: 3074 1791 1143 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3958 4775 4792 4945 5130 5575 325 Size: 1255 2378 817 17 153 185 445 1498 Fragments arranged by size: 2378 1498 1255 817 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2299 2976 3333 Size: 274 80 33 51 1443 322 677 357 Cuts at: 3333 4994 6706 96 Size: 1661 1712 138 Fragments arranged by size: 1712 1661 1443 677 357 322 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6708 6740 6708 Size: 32 6716 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6748 XbaI T'CTAG_A Cuts at: 4084 4084 Size: 6748 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3850 3850 Size: 6748 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6748 XmaI C'CCGG_G Cuts at: 1870 3381 4073 1870 Size: 1511 692 4545 Fragments arranged by size: 4545 1511 692 XmnI GAAnn'nnTTC Cuts at: 4877 4877 Size: 6748 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 3882 4556 276 Size: 53 83 186 936 1837 511 674 2468 Fragments arranged by size: 2468 1837 936 674 511 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI