HUGE

Gene/Protein Characteristic Table for KIAA1973

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01187
Accession No. :	AB075853
Description :	Glutamate [NMDA] receptor subunit 3A precursor.
HUGO Gene Name :
Clone Name :	pf00154 [Vector Info]
Flexi ORF Clone :	pF1KA1973
Source :	Human brain (hippocampus)

Features of the cloned DNA sequence	Description

Length: 7770 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 3821 bp

Genome contig ID gi89161216r_103271456
PolyA signal sequence
(AATAAA,-24)
+----*----+----*----+----*----+----
TATGACTGTGGAATAAATGTTCAGATAGAAACTTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACTTTTGGATGTACTGCTGGCTTTGTCTTTGGGGATTCAAATGTTGACAT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 9 r 103371456 103540683 9 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 1176 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001107918	97.4	similar to glut...	Macaca mulatta
BAG11375	100.0	glutamate [NMDA...	synthetic construct
Q8TCU5	99.9	Glutamate [NMDA...	Homo sapiens
EAW58958	99.7	glutamate recep...	Homo sapiens
CAC95229	99.6	N-methyl D-aspa...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033046	0.0005	24.1	KIAA1220

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

BlastProDom NULL 801 870 PD245531 NULL

FPrintScan IPR001508 654 682 PR00177 NMDA receptor

IPR001508 738 763 PR00177 NMDA receptor

IPR001508 805 832 PR00177 NMDA receptor

IPR001508 992 1016 PR00177 NMDA receptor

HMMPfam IPR001320 735 1013 PF00060 Ionotropic glutamate receptor

HMMSmart IPR001320 626 971 SM00079 Ionotropic glutamate receptor

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	801	870	PD245531	NULL
FPrintScan	IPR001508	654	682	PR00177	NMDA receptor
IPR001508	738	763	PR00177	NMDA receptor
IPR001508	805	832	PR00177	NMDA receptor
IPR001508	992	1016	PR00177	NMDA receptor
HMMPfam	IPR001320	735	1013	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	626	971	SM00079	Ionotropic glutamate receptor

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 69 WLLSRVCLLLPPPCALVLAGVP 90 PRIMARY 22

2 735 HWTMWLGIFVALHITAVFLTLY 756 PRIMARY 22

3 773 KVFSFSSALNICYALLFGRTVAI 795 SECONDARY 23

4 805 FLMNLWAIFCMFCLSTYTANLAA 827 SECONDARY 23

5 990 KHFSGLFVLLCIGFGLSILTTIG 1012 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	69	WLLSRVCLLLPPPCALVLAGVP	90	PRIMARY	22
2	735	HWTMWLGIFVALHITAVFLTLY	756	PRIMARY	22
3	773	KVFSFSSALNICYALLFGRTVAI	795	SECONDARY	23
4	805	FLMNLWAIFCMFCLSTYTANLAA	827	SECONDARY	23
5	990	KHFSGLFVLLCIGFGLSILTTIG	1012	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CAGTGCTGAAGATTATGTGAG

Primer_r
: CACAGTGAGAAGTTTGCAGTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 9

Experimental conditions

Panel name	: genbank
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	YES

Length of 3'UTR	3821 bp
Genome contig ID	gi89161216r_103271456
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- TATGACTGTGGAATAAATGTTCAGATAGAAACTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTTTTGGATGTACTGCTGGCTTTGTCTTTGGGGATTCAAATGTTGACAT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	9	r	103371456	103540683	9	99.4	Perfect prediction

Primer_f	: CAGTGCTGAAGATTATGTGAG
Primer_r	: CACAGTGAGAAGTTTGCAGTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1973

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information