##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
ApaI AscI Asp718I BamHI BbrPI
BcgI BsiWI BsmBI BspDI BspLU11I
ClaI CpoI Eco47III EcoRV FseI
KpnI KspI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
ScaI SfiI SgrAI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3464 GTATAC GT^[AC][GT]AC
AccIII 805 TCCGGA T^CCGGA
AflIII 3595 ACGTGT A^C[GA][CT]GT
AhdI 1699 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 758 GTGCAC G^TGCAC
ApaLI 758 GTGCAC G^TGCAC
Asp700I 5917 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1699 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 662 TGCGCA TGC^GCA
AvrII 3945 CCTAGG C^CTAGG
BbvCI 4049 GCTGAGG CCTCAGC(-5/-2)
BlnI 3945 CCTAGG C^CTAGG
BsaAI 1763 TACGTA [CT]AC^GT[GA]
BsaWI 805 TCCGGA [AT]^CCGG[AT]
BseAI 805 TCCGGA T^CCGGA
BsiEI 247 CGGCCG CG[GA][CT]^CG
BspEI 805 TCCGGA T^CCGGA
BspMI 4431 GCAGGT ACCTGC(4/8)
BsrFI 336 GCCGGC [GA]^CCGG[CT]
BssSI 1277 CACGAG CACGAG(-5/-1)
Bst1107I 3464 GTATAC GTA^TAC
BstBI 1616 TTCGAA TT^CGAA
BstZ17I 3464 GTATAC GTA^TAC
Cfr10I 336 GCCGGC [GA]^CCGG[CT]
EagI 247 CGGCCG C^GGCCG
Eam1105I 1699 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 247 CGGCCG C^GGCCG
Eco52I 247 CGGCCG C^GGCCG
EcoRI 6271 GAATTC G^AATTC
FspI 662 TGCGCA TGC^GCA
MroI 805 TCCGGA T^CCGGA
NaeI 336 GCCGGC GCC^GGC
NgoMIV 336 GCCGGC G^CCGGC
NspV 1616 TTCGAA TT^CGAA
PaeR7I 3845 CTCGAG C^TCGAG
SapI 2924 GAAGAGC GCTCTTC(1/4)
SbfI 52 CCTGCAGG CCTGCA^GG
SfuI 1616 TTCGAA TT^CGAA
SnaBI 1763 TACGTA TAC^GTA
Sse8387I 52 CCTGCAGG CCTGCA^GG
XcmI 138 CCAGGAAATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3845 CTCGAG C^TCGAG
XmnI 5917 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspI 1229 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3621 GACCCCGTC
BciVI 3215 GGATAC GTATCC(6/5)
1602 GTATCC
BclI 1260 TGATCA T^GATCA
6403 TGATCA
BglII 2916 AGATCT A^GATCT
4195 AGATCT
BsaBI 1054 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1261 GATCAGCATC
DraI 4470 TTTAAA TTT^AAA
7013 TTTAAA
DrdI 853 GACTAGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3934 GACTTGTGAGTC
EcoNI 52 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2963 CCTTCCCAAGG
EcoT22I 3038 ATGCAT ATGCA^T
4661 ATGCAT
FbaI 1260 TGATCA T^GATCA
6403 TGATCA
HincII 4228 GTTAAC GT[CT]^[GA]AC
5339 GTTAAC
HindII 4228 GTTAAC GT[CT]^[GA]AC
5339 GTTAAC
HindIII 4971 AAGCTT A^AGCTT
6371 AAGCTT
HpaI 4228 GTTAAC GTT^AAC
5339 GTTAAC
MamI 1054 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1261 GATCAGCATC
NcoI 1115 CCATGG C^CATGG
2016 CCATGG
NsiI 3038 ATGCAT ATGCA^T
4661 ATGCAT
PflFI 1229 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3621 GACCCCGTC
SexAI 4240 ACCAGGT A^CC[AT]GGT
6931 ACCTGGT
SphI 4583 GCATGC GCATG^C
6899 GCATGC
SspI 1893 AATATT AAT^ATT
4255 AATATT
StuI 1069 AGGCCT AGG^CCT
1930 AGGCCT
Tth111I 1229 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3621 GACCCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AseI AsnI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BfrI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BsrBI BsrDI BsrGI BsrI
BssHII BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmaCI XmaI