HUGE

Gene/Protein Characteristic Table for KIAA1529

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB040962
Description :
HUGO Gene Name :
Clone Name :	hh04104 [Vector Info]
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 5752 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
NO NO

Warning for coding interruption:
YES YES

Integrity of 3' end

Length of 3'UTR 393 bp

Genome contig ID gi89161216f_98940600
PolyA signal sequence
(AATAAA,-20)
+----*----+----*----+----*----+----
ATTTTAATTTAACAGAATAAACAAGTATGGGTTAT
Flanking genome sequence
(238792 - 238841)
----+----*----+----*----+----*----+----*----+----*
AATTGCAACTCTGCACTGGTTCCTAGACCCAACAAATAACTAATCCAATG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 9 f 99040600 99179390 48 99.7 Perfect prediction

Features of the protein sequence	Description

Length: 1680 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
NP_065944	100.0	hypothetical pr...	Homo sapiens
CAH18175	87.1	hypothetical pr...	Homo sapiens
XP_520133	85.9	hypothetical pr...	Pan troglodytes
BAG64113	98.9	unnamed protein...	Homo sapiens
CAM22128	63.1	novel protein [...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 77 NVLLGLGACLSRLLISCLLGMWL 99 PRIMARY 23

2 118 GFSAWIGMLYMDHYHINPVLVSF 140 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	77	NVLLGLGACLSRLLISCLLGMWL	99	PRIMARY	23
2	118	GFSAWIGMLYMDHYHINPVLVSF	140	SECONDARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ACTATCCAAGGCCTGTATGTG

Primer_r
: ATGGACGGAAAGTAGGTAGGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 9

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: ACTATCCAAGGCCTGTATGTG
Primer_r	: ATGGACGGAAAGTAGGTAGGC
PCR product length	: 106 bp
PCR conditions	: 95 °C15 sec64 °C60 sec35 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	NO	NO
Warning for coding interruption:	YES	YES

Length of 3'UTR	393 bp
Genome contig ID	gi89161216f_98940600
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- ATTTTAATTTAACAGAATAAACAAGTATGGGTTAT
Flanking genome sequence (238792 - 238841)	----+--------+--------+--------+--------+----* AATTGCAACTCTGCACTGGTTCCTAGACCCAACAAATAACTAATCCAATG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	9	f	99040600	99179390	48	99.7	Perfect prediction

Gene/Protein Characteristic Table for KIAA1529

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information