##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI ApaI AscI AseI AsnI
Asp718I AvrII BbeI BlnI BsaHI
BsiWI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstBI BstZ17I CpoI DraI
DraIII DrdI EcoRV FseI Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 1950 TCCGGA T^CCGGA
AflII 4807 CTTAAG C^TTAAG
AhdI 3914 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 411 CCTGAGG CC^T[ACGT]AGG
AspEI 3914 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1762 TGCGCA TGC^GCA
BfrI 4807 CTTAAG C^TTAAG
BglI 945 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1371 AGATCT A^GATCT
BseAI 1950 TCCGGA T^CCGGA
BsiEI 5196 CGGCCG CG[GA][CT]^CG
BsmBI 1853 CGTCTC CGTCTC(1/5)
BspDI 5158 ATCGAT AT^CGAT
BspEI 1950 TCCGGA T^CCGGA
BspHI 2115 TCATGA T^CATGA
BssSI 253 CTCGTG CACGAG(-5/-1)
BstAPI 5323 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 411 CCTGAGG CC^T[ACGT]AGG
ClaI 5158 ATCGAT AT^CGAT
EagI 5196 CGGCCG C^GGCCG
Eam1105I 3914 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5196 CGGCCG C^GGCCG
Eco52I 5196 CGGCCG C^GGCCG
Eco81I 411 CCTGAGG CC^T[ACGT]AGG
EcoRI 5570 GAATTC G^AATTC
EcoT22I 2546 ATGCAT ATGCA^T
FspI 1762 TGCGCA TGC^GCA
HgaI 1265 GACGC GACGC(5/10)
HincII 2165 GTCAAC GT[CT]^[GA]AC
HindII 2165 GTCAAC GT[CT]^[GA]AC
MluI 1266 ACGCGT A^CGCGT
MroI 1950 TCCGGA T^CCGGA
NcoI 2109 CCATGG C^CATGG
NdeI 310 CATATG CA^TATG
NsiI 2546 ATGCAT ATGCA^T
PaeR7I 1215 CTCGAG C^TCGAG
PshAI 651 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2115 TCATGA T^CATGA
SbfI 663 CCTGCAGG CCTGCA^GG
ScaI 3124 AGTACT AGT^ACT
Sse8387I 663 CCTGCAGG CCTGCA^GG
XhoI 1215 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 733 ACGTGT A^C[GA][CT]GT
1266 ACGCGT
Alw44I 334 GTGCAC G^TGCAC
382 GTGCAC
Aor51HI 1733 AGCGCT AGC^GCT
5301 AGCGCT
ApaLI 334 GTGCAC G^TGCAC
382 GTGCAC
BbvCI 3847 CCTCAGC CCTCAGC(-5/-2)
1382 GCTGAGG
BcgI 710 CGACCATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4184 CGACATCGGTGC
BlpI 4054 GCTGAGC GC^T[ACGT]AGC
5297 GCTCAGC
Bpu1102I 4054 GCTGAGC GC^T[ACGT]AGC
5297 GCTCAGC
BsaBI 2500 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4582 GATCCAGATC
BsaWI 1950 TCCGGA [AT]^CCGG[AT]
2139 ACCGGA
BsmI 523 GCATTC GAATGC(1/-1)
2443 GCATTC
Bsp1407I 2085 TGTACA T^GTACA
5276 TGTACA
BsrFI 1203 ACCGGC [GA]^CCGG[CT]
2835 ACCGGC
BsrGI 2085 TGTACA T^GTACA
5276 TGTACA
BstXI 512 CCATTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4308 CCAACAAAATGG
CelII 4054 GCTGAGC GC^T[ACGT]AGC
5297 GCTCAGC
Cfr10I 1203 ACCGGC [GA]^CCGG[CT]
2835 ACCGGC
DsaI 2109 CCATGG C^C[GA][CT]GG
5199 CCGTGG
Eco47III 1733 AGCGCT AGC^GCT
5301 AGCGCT
EcoT14I 2109 CCATGG C^C[AT][AT]GG
5341 CCAAGG
HaeII 1733 AGCGCT [GA]GCGC^[CT]
5301 AGCGCT
MamI 2500 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4582 GATCCAGATC
SphI 3225 GCATGC GCATG^C
5660 GCATGC
SspBI 2085 TGTACA T^GTACA
5276 TGTACA
SspI 112 AATATT AAT^ATT
5229 AATATT
StuI 4466 AGGCCT AGG^CCT
5344 AGGCCT
StyI 2109 CCATGG C^C[AT][AT]GG
5341 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvI
BciVI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI