HUGE

Gene/Protein Characteristic Table for KIAA1252

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00781
Accession No. :	AB033078
Description :	Sphingosine-1-phosphate lyase 1.
HUGO Gene Name :
Clone Name :	hh09572 [Vector Info]
Flexi ORF Clone :	pF1KSDA1252
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 5741 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3834 bp

Genome contig ID gi89161187f_72145732
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
TTTGGATTAATTTTAGAATAAACCTATTTATTTCT
Flanking genome sequence
(165206 - 165255)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAGAAAAGAAAGTGTTACCTGTGTCTTTGTCTCACATGAAT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 10 f 72245732 72310936 15 99.3 Perfect prediction

Features of the protein sequence	Description

Length: 580 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O95470	100.0	Sphingosine-1-p...	Homo sapiens
XP_521504	99.8	sphingosine-1-p...	Pan troglodytes
CAA09590	99.8	sphingosine-1-p...	Homo sapiens
XP_001171350	99.6	sphingosine-1-p...	Pan troglodytes
Q5R4G0	99.5	Sphingosine-1-p...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR002129 155 468 PF00282 Pyridoxal phosphate-dependent decarboxylase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002129	155	468	PF00282	Pyridoxal phosphate-dependent decarboxylase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 51 PWQLIAWSVVWTLLIVWGYEFVF 73 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	51	PWQLIAWSVVWTLLIVWGYEFVF	73	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCTGCATCACATTACTACACG

Primer_r
: TTCTGCAACCATATTCCTGTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 10

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	3834 bp
Genome contig ID	gi89161187f_72145732
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- TTTGGATTAATTTTAGAATAAACCTATTTATTTCT
Flanking genome sequence (165206 - 165255)	----+--------+--------+--------+--------+----* AAAAAAAAAAAGAAAAGAAAGTGTTACCTGTGTCTTTGTCTCACATGAAT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	10	f	72245732	72310936	15	99.3	Perfect prediction

Primer_f	: TCTGCATCACATTACTACACG
Primer_r	: TTCTGCAACCATATTCCTGTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1252

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information