HUGE

Gene/Protein Characteristic Table for KIAA1123

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK04415
Accession No. :	AB032949
Description :	Voltage-dependent T-type calcium channel subunit alpha-1G.
HUGO Gene Name :
Clone Name :	fh01458 [Vector Info]
Source :	Human fetal brain
Note :	We replaced hj05096, former representative clones for KIAA1123 with fh01458. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5475 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 320 bp

Genome contig ID gi51511734f_45908409
PolyA signal sequence
(ATTAAA,-21)
+----*----+----*----+----*----+----
GTATATTCTATTTTATTAAATTAATTGAATCTAGT
Flanking genome sequence
(151024 - 151073)
----+----*----+----*----+----*----+----*----+----*
ATATGCGGGATGTACGACATTTTGTGACTGAAGAGACTTGTTTCCTTCTA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 17 f 46008409 46059431 28 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 1717 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAF37696	100.0	voltage-depende...	Homo sapiens
AAF37689	99.5	voltage-depende...	Homo sapiens
ABF69908	99.6	T calcium chann...	Homo sapiens
XP_865671	94.4	similar to volt...	Canis lupus fam...
XP_001252667	94.2	calcium channel...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032946	4e-85	54.9	KIAA1120
AB037777	5.3e-11	30.6	KIAA1356

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR005445 212 225 PR01629 T-type voltage-dependent calcium channel alpha 1 subunit

IPR002077 355 381 PR00167 Ca2+ channel

IPR002077 918 930 PR00167 Ca2+ channel

IPR005445 999 1014 PR01629 T-type voltage-dependent calcium channel alpha 1 subunit

HMMPfam IPR005821 228 414 PF00520 Ion transport

IPR005821 763 987 PF00520 Ion transport

IPR005821 1078 1283 PF00520 Ion transport

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR005445	212	225	PR01629	T-type voltage-dependent calcium channel alpha 1 subunit
IPR002077	355	381	PR00167	Ca2+ channel
IPR002077	918	930	PR00167	Ca2+ channel
IPR005445	999	1014	PR01629	T-type voltage-dependent calcium channel alpha 1 subunit
HMMPfam	IPR005821	228	414	PF00520	Ion transport
IPR005821	763	987	PF00520	Ion transport
IPR005821	1078	1283	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 193 SKYFGRGIMIAILVNTLSMGIE 214 SECONDARY 22

2 227 EISNIVFTSLFALEMLLKLLVYG 249 SECONDARY 23

3 256 NPYNIFDGVIVVISVWEIVGQQG 278 SECONDARY 23

4 319 FCMLLMLFIFIFSILGMHLFGCK 341 PRIMARY 23

5 356 NFDSLLWAIVTVFQILTQEDWNK 378 SECONDARY 23

6 394 YFIALMTFGNYVLFNLLVAILVE 416 PRIMARY 23

7 727 KMFDHVVLVIIFLNCITIAMER 748 PRIMARY 22

8 763 LSNYIFTAVFLAEMTVKVVALGW 785 SECONDARY 23

9 804 LVLISVIDILVSMVSDSGTKILG 826 PRIMARY 23

10 865 NIVVICCAFFIIFGILGVQLFK 886 PRIMARY 22

11 922 NLGQALMSLFVLASKDGWVDIMY 944 SECONDARY 23

12 966 LYFISFLLIVAFFVLNMFVGVVV 988 PRIMARY 23

13 1045 YLDLFITGVIGLNVVTMAMEHYQ 1067 SECONDARY 23

14 1077 KICNYIFTVIFVLESVFKLVAFG 1099 SECONDARY 23

15 1107 RWNQLDLAIVLLSIMGITLEEIE 1129 PRIMARY 23

16 1178 NLGLLFMLLFFIFAALGVELFGD 1200 PRIMARY 23

17 1259 ISPIYFVSFVLTAQFVLVNVVIA 1281 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	193	SKYFGRGIMIAILVNTLSMGIE	214	SECONDARY	22
2	227	EISNIVFTSLFALEMLLKLLVYG	249	SECONDARY	23
3	256	NPYNIFDGVIVVISVWEIVGQQG	278	SECONDARY	23
4	319	FCMLLMLFIFIFSILGMHLFGCK	341	PRIMARY	23
5	356	NFDSLLWAIVTVFQILTQEDWNK	378	SECONDARY	23
6	394	YFIALMTFGNYVLFNLLVAILVE	416	PRIMARY	23
7	727	KMFDHVVLVIIFLNCITIAMER	748	PRIMARY	22
8	763	LSNYIFTAVFLAEMTVKVVALGW	785	SECONDARY	23
9	804	LVLISVIDILVSMVSDSGTKILG	826	PRIMARY	23
10	865	NIVVICCAFFIIFGILGVQLFK	886	PRIMARY	22
11	922	NLGQALMSLFVLASKDGWVDIMY	944	SECONDARY	23
12	966	LYFISFLLIVAFFVLNMFVGVVV	988	PRIMARY	23
13	1045	YLDLFITGVIGLNVVTMAMEHYQ	1067	SECONDARY	23
14	1077	KICNYIFTVIFVLESVFKLVAFG	1099	SECONDARY	23
15	1107	RWNQLDLAIVLLSIMGITLEEIE	1129	PRIMARY	23
16	1178	NLGLLFMLLFFIFAALGVELFGD	1200	PRIMARY	23
17	1259	ISPIYFVSFVLTAQFVLVNVVIA	1281	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCTTCATCACAGGTGTCATCG

Primer_r
: ATGATGGACAGCAGCACAATG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 17

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GAGTTAAAAGCAGCAGCCCCG
Primer_r	: TGTCGGGAACCTCAGCTGCAC
PCR product length	: 75 bp
PCR conditions	: 95 °C15 sec68 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	320 bp
Genome contig ID	gi51511734f_45908409
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- GTATATTCTATTTTATTAAATTAATTGAATCTAGT
Flanking genome sequence (151024 - 151073)	----+--------+--------+--------+--------+----* ATATGCGGGATGTACGACATTTTGTGACTGAAGAGACTTGTTTCCTTCTA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	17	f	46008409	46059431	28	100.0	Perfect prediction

Primer_f	: TCTTCATCACAGGTGTCATCG
Primer_r	: ATGATGGACAGCAGCACAATG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1123

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information