##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AatII       AclI        AflII       AocI        AscI        
AspI        AvrII       BfrI        BlnI        BsiWI       
BsmI        Bsp1407I    BspDI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        Eco81I      EcoRI       EcoRV       
FseI        HindIII     HpaI        KspI        MluI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PflFI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SacII       SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       5293  GGTACC               G^GTACC
AccI         1319  GTCGAC               GT^[AC][GT]AC
AccIII       5013  TCCGGA               T^CCGGA
AcsI         1066  GAATTT               [GA]^AATT[CT]
AgeI         2733  ACCGGT               A^CCGGT
ApoI         1066  GAATTT               [GA]^AATT[CT]
AseI         5460  ATTAAT               AT^TAAT
AsnI         5460  ATTAAT               AT^TAAT
Asp700I      2662  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      5293  GGTACC               G^GTACC
AviII        3441  TGCGCA               TGC^GCA
BbrPI        2192  CACGTG               CAC^GTG
BclI         2526  TGATCA               T^GATCA
BsaBI         604  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        5013  TCCGGA               T^CCGGA
BspEI        5013  TCCGGA               T^CCGGA
BstEII       3175  GGTCACC              G^GT[ACGT]ACC
BstPI        3175  GGTCACC              G^GT[ACGT]ACC
DraIII       5187  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      3175  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1004  ATGCAT               ATGCA^T
FbaI         2526  TGATCA               T^GATCA
FspI         3441  TGCGCA               TGC^GCA
KpnI         5293  GGTACC               GGTAC^C
MamI          604  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3706  CAATTG               C^AATTG
MroI         5013  TCCGGA               T^CCGGA
MunI         3706  CAATTG               C^AATTG
NsiI         1004  ATGCAT               ATGCA^T
PaeR7I       2099  CTCGAG               C^TCGAG
PinAI        2733  ACCGGT               A^CCGGT
PmaCI        2192  CACGTG               CAC^GTG
PmlI         2192  CACGTG               CAC^GTG
PshBI        5460  ATTAAT               AT^TAAT
SalI         1319  GTCGAC               G^TCGAC
ScaI          582  AGTACT               AGT^ACT
SmlI         2099  CTCGAG               C^T[CT][GA]AG
SpeI          172  ACTAGT               A^CTAGT
XhoI         2099  CTCGAG               C^TCGAG
XmnI         2662  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AflIII       2943  ACATGT               A^C[GA][CT]GT
             4176  ACATGT              
AhdI         2108  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3746  GACCAGGAGTC         
Aor51HI      1646  AGCGCT               AGC^GCT
             2264  AGCGCT              
AspEI        2108  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3746  GACCAGGAGTC         
BamHI        2070  GGATCC               G^GATCC
             5044  GGATCC              
BcgI          630  GCATGGGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2223  GCATCACCATCG        
BciVI        2686  GGATAC               GTATCC(6/5)
              121  GTATCC              
BlpI         3905  GCTGAGC              GC^T[ACGT]AGC
             4681  GCTGAGC             
Bpu1102I     3905  GCTGAGC              GC^T[ACGT]AGC
             4681  GCTGAGC             
BsaAI        1211  TACGTG               [CT]AC^GT[GA]
             2192  CACGTG              
BsmBI         152  GAGACG               CGTCTC(1/5)
             2561  GAGACG              
BspLU11I     2943  ACATGT               A^CATGT
             4176  ACATGT              
BssSI         647  CACGAG               CACGAG(-5/-1)
             1595  CACGAG              
CelII        3905  GCTGAGC              GC^T[ACGT]AGC
             4681  GCTGAGC             
EagI         1482  CGGCCG               C^GGCCG
             1693  CGGCCG              
Eam1105I     2108  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3746  GACCAGGAGTC         
EclXI        1482  CGGCCG               C^GGCCG
             1693  CGGCCG              
Eco47III     1646  AGCGCT               AGC^GCT
             2264  AGCGCT              
Eco52I       1482  CGGCCG               C^GGCCG
             1693  CGGCCG              


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaI        ApaLI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DrdI        DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SphI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI