HUGE

Gene/Protein Characteristic Table for KIAA1094

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00739
Accession No. :	AB029017
Description :	Transmembrane protein 15.
HUGO Gene Name :	dolichol kinase (DOLK)
Clone Name :	hk06464a [Vector Info]
Flexi ORF Clone :	pF1KSDA1094
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 2089 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 156 bp

Genome contig ID gi89161216r_130647631
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
TGATTCAGATTTAAAATAAAAAGCAAAGCTCTCCT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGTTCTAGTTTTGCTGATATATTTTTTTTTTTATTTTTTTTTGAGACAGT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 9 r 130747631 130749719 1 99.0 Perfect prediction

Features of the protein sequence	Description

Length: 544 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9UPQ8	1.9e-206	100.0	Dolichol kinase...	Homo sapiens
ABY56110	1.1e-205	99.6	dolichol kinase...	Papio anubis
BAE91315	3.3e-205	99.4	unnamed protein...	Macaca fascicularis
ACA57898	1.6e-204	98.9	transmembrane p...	Callicebus moloch
ABY84169	1.3e-202	98.1	transmembrane p...	Callithrix jacchus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR000374 341 543 PF01148 Phosphatidate cytidylyltransferase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000374	341	543	PF01148	Phosphatidate cytidylyltransferase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 22 LSGSVLAEAAVVFAVVLSIHATV 44 PRIMARY 23

2 114 ERFGIVVAATGMAVALFSSVLAL 136 PRIMARY 23

3 142 VPTNTCVILGLAGGVIIYIMKHS 164 PRIMARY 23

4 170 VIEVLEVLLIFVYLNMILLYLLP 192 PRIMARY 23

5 194 CFTPGEALLVLGGISFVLNQLI 215 SECONDARY 22

6 229 VDFFLLVVVVGMVLMGIFFSTLF 251 PRIMARY 23

7 264 FFHLMTCVLSLGVVLPWLHRLI 285 SECONDARY 22

8 305 YLLAYWSLLATLACLVVLYQNA 326 PRIMARY 22

9 352 ATYIPGIIFDRPLLYVAATVCLA 374 PRIMARY 23

10 408 GPLILTHIYLLLGMSLPIWLIPR 430 SECONDARY 23

11 442 RALVPYAGVLAVGVGDTVASIFG 464 SECONDARY 23

12 481 GTMTSIFAQIISVALILIFDSGV 503 SECONDARY 23

13 516 ISTVSLLEAYTTQIDNLLLPLYL 538 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	LSGSVLAEAAVVFAVVLSIHATV	44	PRIMARY	23
2	114	ERFGIVVAATGMAVALFSSVLAL	136	PRIMARY	23
3	142	VPTNTCVILGLAGGVIIYIMKHS	164	PRIMARY	23
4	170	VIEVLEVLLIFVYLNMILLYLLP	192	PRIMARY	23
5	194	CFTPGEALLVLGGISFVLNQLI	215	SECONDARY	22
6	229	VDFFLLVVVVGMVLMGIFFSTLF	251	PRIMARY	23
7	264	FFHLMTCVLSLGVVLPWLHRLI	285	SECONDARY	22
8	305	YLLAYWSLLATLACLVVLYQNA	326	PRIMARY	22
9	352	ATYIPGIIFDRPLLYVAATVCLA	374	PRIMARY	23
10	408	GPLILTHIYLLLGMSLPIWLIPR	430	SECONDARY	23
11	442	RALVPYAGVLAVGVGDTVASIFG	464	SECONDARY	23
12	481	GTMTSIFAQIISVALILIFDSGV	503	SECONDARY	23
13	516	ISTVSLLEAYTTQIDNLLLPLYL	538	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CTCTACCTCCTGATATTGCTG

Primer_r
: TCACACCTTGGCTCTTCATGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 9

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CTCTACCTCCTGATATTGCTG
Primer_r	: TCACACCTTGGCTCTTCATGC
PCR product length	: 129 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	156 bp
Genome contig ID	gi89161216r_130647631
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TGATTCAGATTTAAAATAAAAAGCAAAGCTCTCCT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTTCTAGTTTTGCTGATATATTTTTTTTTTTATTTTTTTTTGAGACAGT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	9	r	130747631	130749719	1	99.0	Perfect prediction

Gene/Protein Characteristic Table for KIAA1094

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information