##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KpnI        
MfeI        MluI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SalI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SmlI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tsp509I     
Tth111I     Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII          95  GACGTC               GACGT^C
AccI           98  GTCTAC               GT^[AC][GT]AC
AccII           6  CGCG                 CG^CG
Aor51HI       945  AGCGCT               AGC^GCT
AviII        1759  TGCGCA               TGC^GCA
BalI         1236  TGGCCA               TGG^CCA
BbeI          135  GGCGCC               GGCGC^C
BbvCI         249  GCTGAGG              CCTCAGC(-5/-2)
BsaBI        1783  GATCTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1227  GGTCTC               GGTCTC(1/5)
BsmI         1270  GAATGC               GAATGC(1/-1)
BsrBI         605  GAGCGG               CCGCTC(-3/-3)
BssSI         442  CTCGTG               CACGAG(-5/-1)
BstUI           6  CGCG                 CG^CG
BstYI        1704  AGATCC               [GA]^GATC[CT]
DraI         2064  TTTAAA               TTT^AAA
Eco47III      945  AGCGCT               AGC^GCT
FspI         1759  TGCGCA               TGC^GCA
HgaI          271  GCGTC                GACGC(5/10)
KasI          135  GGCGCC               G^GCGCC
KspI            5  CCGCGG               CCGC^GG
MamI         1783  GATCTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1704  AGATCC               [GA]^GATC[CT]
MluNI        1236  TGGCCA               TGG^CCA
MscI         1236  TGGCCA               TGG^CCA
MvnI            6  CGCG                 CG^CG
NaeI           48  GCCGGC               GCC^GGC
NarI          135  GGCGCC               GG^CGCC
NgoMIV         48  GCCGGC               G^CCGGC
NspI         1557  GCATGT               [GA]CATG^[CT]
PpuMI         975  GGGACCC              [GA]G^G[AT]CC[CT]
PstI           83  CTGCAG               CTGCA^G
SacII           5  CCGCGG               CCGC^GG
SapI         2016  GAAGAGC              GCTCTTC(1/4)
SfiI         1237  GGCCACCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          135  GGCGCC               GGC^GCC
SmaI          343  CCCGGG               CCC^GGG
SpeI          592  ACTAGT               A^CTAGT
TaqI          810  TCGA                 T^CGA
TthHB8I       810  TCGA                 T^CGA
XbaI          308  TCTAGA               T^CTAGA
XcmI         1404  CCACTGTATGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1704  AGATCC               [GA]^GATC[CT]
XmaCI         343  CCCGGG               C^CCGGG
XmaI          343  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwNI         660  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1585  CAGACCCTG           
ApaI          255  GGGCCC               GGGCC^C
             1623  GGGCCC              
Asp700I       625  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              821  GAAGTCCTTC          
AvaI          321  CCCGAG               C^[CT]CG[GA]G
              343  CCCGGG              
BglI           17  GCCAAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1238  GCCACCTTGGC         
BlpI          409  GCTGAGC              GC^T[ACGT]AGC
             1111  GCTGAGC             
Bpu1102I      409  GCTGAGC              GC^T[ACGT]AGC
             1111  GCTGAGC             
BsoBI         321  CCCGAG               C^[CT]CG[GA]G
              343  CCCGGG              
BstXI         613  CCAGACTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              663  CCACTGGCATGG        
CelII         409  GCTGAGC              GC^T[ACGT]AGC
             1111  GCTGAGC             
MaeII          96  ACGT                 A^CGT
              480  ACGT                
MseI         1787  TTAA                 T^TAA
             2065  TTAA                
NcoI          570  CCATGG               C^CATGG
             1695  CCATGG              
PleI         1061  GACTC                GAGTC(4/5)
             1292  GAGTC               
TfiI         1159  GAATC                G^A[AT]TC
             2056  GATTC               
Tru9I        1787  TTAA                 T^TAA
             2065  TTAA                
XmnI          625  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              821  GAAGTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        Tsp45I      TspRI