HUGE

Gene/Protein Characteristic Table for KIAA1089

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00736
Accession No. :	AB029012
Description :	Protein SMG5.
HUGO Gene Name :	Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG5)
Clone Name :	ha02541 [Vector Info]
Flexi ORF Clone :	pF1KSDA1089
Source :	Myeloblast cell line (KG-1)
Note :	We replaced hk03401, former representative clones for KIAA1089 with ha02541. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 4563 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1361 bp

Genome contig ID gi89161185r_154385641
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
AGACTAAAGCATCACTTAATAAACCCCCCAAGCCC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AATCCCTGTCTCCTGGTGCCTGTCTGTACAGAAGTTTCATTGGGGGGTGG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 154485641 154519233 22 99.6 Terminal No-hit

Features of the protein sequence	Description

Length: 1033 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_513880	99.6	Est1p-like prot...	Pan troglodytes
Q9UPR3	100.0	Protein SMG5; S...	Homo sapiens
AAH38296	99.9	Smg-5 homolog, ...	Homo sapiens
AAQ84301	99.7	LPTS interactin...	Homo sapiens
AAI34681	96.2	SMG5 protein [B...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D87437	7.1e-07	25.0	KIAA0250
AB018275	1.7e-05	24.8	KIAA0732

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMSmart IPR006596 872 995 SM00670 Nucleotide binding protein

Search method	interpro_ID	From	To	Entry	Definition
HMMSmart	IPR006596	872	995	SM00670	Nucleotide binding protein

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 365 AFLPDLLIFQMVIICLMCVHSLE 387 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	365	AFLPDLLIFQMVIICLMCVHSLE	387	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GGAGCAGAGATCATGAATAGC

Primer_r
: GCCAACTATCCTCTAAGCCAC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GGAGCAGAGATCATGAATAGC
Primer_r	: GCCAACTATCCTCTAAGCCAC
PCR product length	: 211 bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1361 bp
Genome contig ID	gi89161185r_154385641
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- AGACTAAAGCATCACTTAATAAACCCCCCAAGCCC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AATCCCTGTCTCCTGGTGCCTGTCTGTACAGAAGTTTCATTGGGGGGTGG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	154485641	154519233	22	99.6	Terminal No-hit

Gene/Protein Characteristic Table for KIAA1089

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information