##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI AspEI
BbeI BbrPI BciVI BclI BfrI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI Bsp1407I BspEI BspLU11I BsrGI
BssHII BssSI BstBI BstUI CpoI
Eam1105I EcoRI EcoT22I FbaI FseI
Hin1I HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 1247 GTGCAC G^TGCAC
Aor51HI 1884 AGCGCT AGC^GCT
ApaLI 1247 GTGCAC G^TGCAC
AspI 1750 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3911 CCTAGG C^CTAGG
BcgI 3408 CGAGGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3911 CCTAGG C^CTAGG
BsmBI 79 GAGACG CGTCTC(1/5)
Bst1107I 407 GTATAC GTA^TAC
BstZ17I 407 GTATAC GTA^TAC
DraI 2888 TTTAAA TTT^AAA
DraIII 359 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2049 GACGCTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1062 CCGTGG C^C[GA][CT]GG
Eco47III 1884 AGCGCT AGC^GCT
EcoRV 699 GATATC GAT^ATC
HaeII 1884 AGCGCT [GA]GCGC^[CT]
HincII 1334 GTCAAC GT[CT]^[GA]AC
HindII 1334 GTCAAC GT[CT]^[GA]AC
MaeII 3396 ACGT A^CGT
NspI 659 GCATGT [GA]CATG^[CT]
PflFI 1750 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 3124 CCTGCAGG CCTGCA^GG
SfiI 1811 GGCCCACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3124 CCTGCAGG CCTGCA^GG
Tth111I 1750 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2871 GGTACC G^GTACC
3504 GGTACC
AccI 407 GTATAC GT^[AC][GT]AC
1985 GTAGAC
Asp700I 2125 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3522 GAATGGGTTC
Asp718I 2871 GGTACC G^GTACC
3504 GGTACC
AviII 2176 TGCGCA TGC^GCA
2442 TGCGCA
BamHI 1268 GGATCC G^GATCC
3043 GGATCC
BglII 2284 AGATCT A^GATCT
3238 AGATCT
BsaI 3946 GGTCTC GGTCTC(1/5)
4372 GGTCTC
BsiEI 35 CGGCCG CG[GA][CT]^CG
73 CGGCCG
BspDI 4 ATCGAT AT^CGAT
2816 ATCGAT
BspHI 2622 TCATGA T^CATGA
4242 TCATGA
BsrBI 2366 CCGCTC CCGCTC(-3/-3)
2942 GAGCGG
BstEII 1755 GGTCACC G^GT[ACGT]ACC
3058 GGTGACC
BstPI 1755 GGTCACC G^GT[ACGT]ACC
3058 GGTGACC
ClaI 4 ATCGAT AT^CGAT
2816 ATCGAT
EagI 35 CGGCCG C^GGCCG
73 CGGCCG
EclXI 35 CGGCCG C^GGCCG
73 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
73 CGGCCG
EcoO65I 1755 GGTCACC G^GT[ACGT]ACC
3058 GGTGACC
FauI 2365 CCCGC CCCGC(4/6)
2481 CCCGC
FspI 2176 TGCGCA TGC^GCA
2442 TGCGCA
KpnI 2871 GGTACC GGTAC^C
3504 GGTACC
NaeI 3361 GCCGGC GCC^GGC
4097 GCCGGC
NgoMIV 3361 GCCGGC G^CCGGC
4097 GCCGGC
NheI 736 GCTAGC G^CTAGC
1832 GCTAGC
PpuMI 2091 AGGTCCT [GA]G^G[AT]CC[CT]
3377 GGGTCCC
PshAI 580 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1643 GACAAGAGTC
RcaI 2622 TCATGA T^CATGA
4242 TCATGA
SacI 332 GAGCTC GAGCT^C
2182 GAGCTC
SapI 3632 GAAGAGC GCTCTTC(1/4)
2348 GCTCTTC
ScaI 825 AGTACT AGT^ACT
4398 AGTACT
SexAI 492 ACCTGGT A^CC[AT]GGT
3647 ACCTGGT
Tsp509I 711 AATT ^AATT
3193 AATT
XmnI 2125 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3522 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI