HUGE

Gene/Protein Characteristic Table for KIAA0686

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK05615
Accession No. :	AB014586
Description :	G-protein coupled receptor 98 precursor.
HUGO Gene Name :	G protein-coupled receptor 98 (GPR98)
Clone Name :	fj02322 [Vector Info]
Source :	Human fetal brain
Note :	We replaced hk02975, former representative clones for KIAA0686 with fj02322. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 3918 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 315 bp

Genome contig ID gi51511721f_90042152
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
TAATGAAAGTAATAATCAATAAAGCAATAGAATCT
Flanking genome sequence
(453638 - 453687)
----+----*----+----*----+----*----+----*----+----*
ATTTGGTATCATCCAGCTTCATTATTGATAAAGAAACATTGTGCATGGGC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 5 f 90142152 90495788 17 99.7 Perfect prediction

Features of the protein sequence	Description

Length: 1200 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAD55586	100.0	very large G-pr...	Homo sapiens
EAW95987	100.0	monogenic, audi...	Homo sapiens
EAW95992	100.0	monogenic, audi...	Homo sapiens
Q8WXG9	100.0	G-protein coupl...	Homo sapiens
EAW95993	100.0	monogenic, audi...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018311	3.3e-14	22.5	KIAA0768
AB018329	1.1e-13	23.0	KIAA0786
AB020628	3.6e-13	23.9	KIAA0821
D87469	8.8e-09	22.5	KIAA0279

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR003644 187 227 PF03160 Na-Ca exchanger/integrin-beta4

IPR000203 746 791 PF01825 GPS

ProfileScan IPR000203 747 796 PS50221 GPS

IPR000832 799 1050 PS50261 GPCR

ScanRegExp IPR003016 292 321 PS00189 2-oxo acid dehydrogenase

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003644	187	227	PF03160	Na-Ca exchanger/integrin-beta4
IPR000203	746	791	PF01825	GPS
ProfileScan	IPR000203	747	796	PS50221	GPS
IPR000832	799	1050	PS50261	GPCR
ScanRegExp	IPR003016	292	321	PS00189	2-oxo acid dehydrogenase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 801 EAFFTSGFICISGLCLAVLSHIF 823 SECONDARY 23

2 833 KLLTHMMAASLGTQILFLASAYA 855 SECONDARY 23

3 869 AVTHYLYLCQFSWMLIQSVNFWY 891 SECONDARY 23

4 906 LFFLLSWGLPAFVVILLIVILKG 928 PRIMARY 23

5 949 PNVYAALFTAALVPLTCLVVVFV 971 PRIMARY 23

6 997 AEIPLILYLFALISVTWLWGGLH 1019 PRIMARY 23

7 1028 LVLFVIFNSLQGLYVFMVYFILH 1050 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	801	EAFFTSGFICISGLCLAVLSHIF	823	SECONDARY	23
2	833	KLLTHMMAASLGTQILFLASAYA	855	SECONDARY	23
3	869	AVTHYLYLCQFSWMLIQSVNFWY	891	SECONDARY	23
4	906	LFFLLSWGLPAFVVILLIVILKG	928	PRIMARY	23
5	949	PNVYAALFTAALVPLTCLVVVFV	971	PRIMARY	23
6	997	AEIPLILYLFALISVTWLWGGLH	1019	PRIMARY	23
7	1028	LVLFVIFNSLQGLYVFMVYFILH	1050	PRIMARY	23

Expression profile	Description

: RT-PCR Description
: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GTGCCTCCATTTCTTCTGCTC

Primer_r
: AGGCAAAGTAAAGGCTCACAG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR	Description

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 5

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GTGCCTCCATTTCTTCTGCTC
Primer_r	: AGGCAAAGTAAAGGCTCACAG
PCR product length	: 164 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	315 bp
Genome contig ID	gi51511721f_90042152
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TAATGAAAGTAATAATCAATAAAGCAATAGAATCT
Flanking genome sequence (453638 - 453687)	----+--------+--------+--------+--------+----* ATTTGGTATCATCCAGCTTCATTATTGATAAAGAAACATTGTGCATGGGC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	5	f	90142152	90495788	17	99.7	Perfect prediction

Gene/Protein Characteristic Table for KIAA0686

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information