HUGE

Gene/Protein Characteristic Table for KIAA0108

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00411
Accession No. :	D14696
Description :	Lysosomal-associated transmembrane protein 4A.
HUGO Gene Name :	lysosomal protein transmembrane 4 alpha (LAPTM4A)
Clone Name :	ha00623 [Vector Info]
Flexi ORF Clone :	pF1KSDA0108
Source :	Myeloblast cell line (KG-1)

Features of the cloned DNA sequence	Description

Length: 1402 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 554 bp

Genome contig ID gi89161199r_19995893
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
TGATAAGATGATTTAATAAATCTCTTTAAATACTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGGGTTGTCATTGTTTTTAATCCGTTACTTTTTTTTAAGATAAACCCTGA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 2 r 20095893 20114908 7 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 254 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q15012	9.6e-96	100.0	Lysosomal-assoc...	Homo sapiens
AAH03158	1.8e-95	99.6	Lysosomal prote...	Homo sapiens
Q4R4I5	3.7e-95	99.1	Lysosomal-assoc...	Macaca fascicularis
Q6QRN8	1.1e-94	98.3	Lysosomal-assoc...	Bos taurus
Q5EI37	2.6e-94	97.9	Lysosomal-assoc...	Gekko japonicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR004687 58 254 PF03821 Golgi 4-transmembrane spanning transporter

HMMTigr IPR004687 31 254 TIGR00799 Golgi 4-transmembrane spanning transporter

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004687	58	254	PF03821	Golgi 4-transmembrane spanning transporter
HMMTigr	IPR004687	31	254	TIGR00799	Golgi 4-transmembrane spanning transporter

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 50 TIILGTWYMVVNLLMAILLTVEV 72 PRIMARY 23

2 98 ADNACVLFAVSVLMFIISSMLVY 120 PRIMARY 23

3 130 LIPFFCYRLFDFVLSCLVAISSL 152 PRIMARY 23

4 181 CLLFIVLVFFALFIIFKAYLINC 203 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	50	TIILGTWYMVVNLLMAILLTVEV	72	PRIMARY	23
2	98	ADNACVLFAVSVLMFIISSMLVY	120	PRIMARY	23
3	130	LIPFFCYRLFDFVLSCLVAISSL	152	PRIMARY	23
4	181	CLLFIVLVFFALFIIFKAYLINC	203	PRIMARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 2

Experimental conditions

Panel name	: Genebridge 4
Primer_f	: TTTGTTACTTGCTACCCTGAG
Primer_r	: CAACCATCATCTTCCACAGTC
PCR product length	: 148 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	554 bp
Genome contig ID	gi89161199r_19995893
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TGATAAGATGATTTAATAAATCTCTTTAAATACTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGGGTTGTCATTGTTTTTAATCCGTTACTTTTTTTTAAGATAAACCCTGA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	2	r	20095893	20114908	7	100.0	Perfect prediction

Gene/Protein Characteristic Table for KIAA0108

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information