Gene/Protein Characteristic Table for KIAA0899
Order Kazusa clone(s) from : Japan || Other countries
Product ID ORK00678
Accession No AB020706
Description adaptor-related protein complex 2, alpha 2 subunit, transcript variant 2
Clone name hk09548s1
Vector information
The cDNA fragment was originally inserted at SalI-NotI site ...
cDNA sequence DNA sequence (4434 bp)
Predicted protein sequence (939 aa)
Flexi ORF Clone FXC00678
Source Human adult brain
Rouge ID mKIAA0899 by Kazusa Mouse cDNA Project
Note We replaced hk09548, former representative clones for KIAA0899 with hk09548s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 4434 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1614 bp
Genome contig ID gi51511727f_816022
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
ACTCCTCGTCAAAGAAAAATAAAGGCTAGAACTGC
Flanking genome sequence
(186219 - 186268)
----+----*----+----*----+----*----+----*----+----*
ACCCCGGATCACGCGCTTTCTTTGGGGGGAAAGCATCCCATGTAACCCTC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 11 f 916022 1002239 22 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 939 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAD97278 0 99.9 adaptor-related...
Homo sapiens
BAH13015 0 99.9 unnamed protein...
Homo sapiens
CAH90132 0 98.9 hypothetical pr...
Pongo abelii
BAC40392 0 97.4 unnamed protein...
Mus musculus
AAH58099 0 97.3 Adaptor protein...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR002553 29 590 PF01602 Clathrin/coatomer adaptor
IPR008152 707 820 PF02883 Clathrin adaptor
IPR003164 826 938 PF02296 Clathrin adaptor
HMMSmart IPR008152 707 820 SM00809 Clathrin adaptor
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AATGTCTGCGGCTCTTCTTCC
Primer_r TGAGAATGAGGGCCACACCAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name GeneBridge 4
Primer_f AATGTCTGCGGCTCTTCTTCC
Primer_r TGAGAATGAGGGCCACACCAC
PCR product length 164 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
Order Kazusa clone(s) from : Japan || Other countries
Back to the HUGE Protein Database homepage
Send a message to office AT kazusa.or.jp