Gene/Protein Characteristic Table for KIAA0851
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Product ID ORK00658
Accession No AB020658
Description SAC1 suppressor of actin mutations 1-like (yeast)
Clone name hk06032
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (3572 bp)
Predicted protein sequence (607 aa)
Flexi ORF Clone FXC00658
Source Human adult brain
Rouge ID mKIAA0851 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 3572 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1739 bp
Genome contig ID gi89161205f_45605933
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
TCCATAAGTGAAAATAAAATGTCATATTCTTTTCT
Flanking genome sequence
(155973 - 156022)
----+----*----+----*----+----*----+----*----+----*
ATTATTGGTATCATTCATGTACTTTGAAAGTGATGTTTTTCTTTTTGATC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 f 45705893 45761904 20 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 607 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9NTJ5 0 100.0 Phosphatidylino...
Homo sapiens
Q5R921 0 99.8 Phosphatidylino...
Pongo abelii
AAH16559 0 99.8 SAC1 suppressor...
Homo sapiens
BAF83831 0 99.7 unnamed protein...
Homo sapiens
CAB66765 0 99.7 hypothetical pr...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB023183 2.1e-53 38.0 KIAA0966
AB020717 2.7e-36 30.6 KIAA0910
AB002346 3.5e-33 30.9 KIAA0348
D87464 5e-11 28.4 KIAA0274
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR002013 77 372 PF02383 Synaptojanin
ProfileScan IPR002013 142 471 PS50275 Synaptojanin

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 541 FLALPIIMVVAFSMCIICLLMAG 563 PRIMARY 23
2 574 LFWGVASIGTFFIILYNGKDFVD 596 SECONDARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TAGCTGGAAAACCCTCTGTAG
Primer_r CATTCCCTACAAAGCAGCCAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name UniGene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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