Gene/Protein Characteristic Table for KIAA0782
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Product ID ORK00637
Accession No AB018325
Description ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1, transcript variant 1
Clone name hk05405s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4919 bp)
Predicted protein sequence (1281 aa)
Flexi ORF Clone FXC00637
Source Human adult brain
Rouge ID mKIAA0782 by Kazusa Mouse cDNA Project
Note We replaced hk05405, former representative clones for KIAA0782 with hk05405s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 4919 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 589 bp
Genome contig ID gi51511727r_71973768
PolyA signal sequence
(TATAAA,-22)
+----*----+----*----+----*----+----
GACTTTTTGATAATATAAATATATCTGTATATTTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACCCTATGGTGCTGAGGCCTGTGATTGGCTAAGGGGATTGGGCGTCCTGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 11 r 72073768 72111028 33 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 1281 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAT36325 0 100.0 ARAP1b protein ...
Homo sapiens
XP_508625 0 99.6 centaurin delta...
Pan troglodytes
XP_001114962 0 98.0 similar to cent...
Macaca mulatta
EAW74863 0 98.9 centaurin, delt...
Homo sapiens
BAG09846 0 100.0 centaurin-delta...
synthetic construct
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB011152 2.5e-68 41.7 KIAA0580
AB040934 6.7e-05 25.7 KIAA1501
D79989 8.2e-05 30.2 KIAA0167
AB051509 0.00013 36.2 KIAA1722
AB014521 0.00031 29.3 KIAA0621
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001164 378 397 PR00405 Arf GTPase activating protein
IPR001164 397 414 PR00405 Arf GTPase activating protein
IPR001164 420 441 PR00405 Arf GTPase activating protein
HMMPfam IPR001849 159 250 PF00169 Pleckstrin-like
IPR001164 366 490 PF01412 Arf GTPase activating protein
IPR001849 575 681 PF00169 Pleckstrin-like
IPR000198 802 951 PF00620 RhoGAP
IPR000159 1003 1092 PF00788 Ras-association
IPR001849 1106 1227 PF00169 Pleckstrin-like
HMMSmart IPR001849 159 252 SM00233 Pleckstrin-like
IPR001849 272 362 SM00233 Pleckstrin-like
IPR001164 366 492 SM00105 Arf GTPase activating protein
IPR001849 575 683 SM00233 Pleckstrin-like
IPR001849 693 787 SM00233 Pleckstrin-like
IPR000198 799 975 SM00324 RhoGAP
IPR001849 1106 1229 SM00233 Pleckstrin-like
ProfileScan IPR001849 158 250 PS50003 Pleckstrin-like
IPR001849 271 360 PS50003 Pleckstrin-like
IPR001164 366 491 PS50115 Arf GTPase activating protein
IPR001849 574 681 PS50003 Pleckstrin-like
IPR000198 785 970 PS50238 RhoGAP
IPR000159 1003 1092 PS50200 Ras-association
IPR001849 1105 1227 PS50003 Pleckstrin-like
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CTGGAGAGATGGGCATAAGTC
Primer_r GCTGTTCTGGAGTTTGTTGGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name GeneBridge 4
Primer_f CTGGAGAGATGGGCATAAGTC
Primer_r GCTGTTCTGGAGTTTGTTGGG
PCR product length 118 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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