Gene/Protein Characteristic Table for KIAA1135
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Product ID ORK00747
Accession No AB032961
Description spire-type actin nucleation factor 1, transcript variant 1
Clone name hj05037
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5495 bp)
Predicted protein sequence (789 aa)
Flexi ORF Clone FXC00747
Source Human adult brain
Rouge ID mKIAA1135 by Kazusa Mouse cDNA Project
Note We replaced hj02644, former representative clones for KIAA1135 with hj05037. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 5495 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 3125 bp
Genome contig ID gi51511735r_12336512
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
AAAATTTAGAGAAAAATAAAATGGCTTTCCATCAG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACTTTCTGTGTTGAGTTGCTATCGTAAGACGGTGATGTCTTTTGGTAATT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 18 r 12436512 12647964 17 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 789 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAG09977 0 100.0 spire homolog 1...
synthetic construct
Q08AE8 0 99.9 Protein spire h...
Homo sapiens
XP_547684 4.7e-210 94.5 similar to spir...
Canis lupus fam...
AAI25207 1.6e-208 100.0 SPIRE1 protein ...
Homo sapiens
AAH58669 2.5e-196 92.6 SPIRE1 protein ...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB058735 1.1e-54 42.9 KIAA1832
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMSmart IPR011019 73 264 SM00750 KIND
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f GCATTACGATCACCTCTTACC
Primer_r ACTTTAACTGTGCCATGTGCC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 18
Experimental conditions
Panel name unigene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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