KIAA0422

Product ID	ORK00533
Accession No	AB007882
Description	adenylate cyclase 6
Clone name	hh01205s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5877 bp) Predicted protein sequence (1160 aa)
HaloTag ORF Clone	FHC00533
Flexi ORF Clone	FXC00533
Source	Human adult brain
Rouge ID	mKIAA0422 by Kazusa Mouse cDNA Project
Note	We replaced hh01205, former representative clones for KIAA0422 with hh01205s1. (2002/5/10)

Length: 5877 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2393 bp
Genome contig ID	gi89161190r_47346248
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- ATATATTTAAATATCAATAAAATCAAACTCTTTGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAATTCTCTGTTCTCTGTAGCTGTTCTGGGCCCTGGGTGGGTCAGAAGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	r	47446248	47469087	21	100.0	Perfect prediction

Length: 1160 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW58001	100.0	adenylate cycla...	Homo sapiens
XP_001102392	99.2	similar to aden...	Macaca mulatta
XP_863292	96.6	similar to aden...	Canis lupus fam...
XP_587001	95.5	adenylate cycla...	Bos taurus
O43306	95.5	Adenylate cycla...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB011083	7.7e-46	40.4	KIAA0511
AB028983	2.7e-37	42.4	KIAA1060
D25538	5.3e-37	39.1	KIAA0037
AB011092	5.3e-28	28.9	KIAA0520

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001054	415	599	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
	IPR009398	605	714	PF06327	Adenylate cyclase-like
	IPR001054	962	1156	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	378	577	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	931	1139	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	424	551	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	971	1110	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	528	551	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	1087	1110	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	195	SSLTLLMAVLVLLTAVLLAFHA	216	PRIMARY	22
	2	226	VALLACAAALFVGLMVVCNRHSF	248	PRIMARY	23
	3	253	MWVVSYVVLGILAAVQVGGALAA	275	PRIMARY	23
	4	284	LWCPVFFVYIAYTLLPIR	301	PRIMARY	18
	5	333	QLGANVLLFLCTNVIGICTHYP	354	SECONDARY	22
	6	713	RFGAYVACALLVFCFICFIQLLI	735	PRIMARY	23
	7	744	GIYASIFLLLLITVLICAVYSCG	766	PRIMARY	23
	8	794	SVLLVFTSAIANMYFIGNMLLSL	816	PRIMARY	23
	9	832	AMIFVLGLIYLVLLLLGPPATIF	854	PRIMARY	23
	10	886	LKYMTPVILLVFALALYLHAQQV	908	PRIMARY	23

Primer_f	ATGGGGTAGGGGTGAGGGTAT
Primer_r	GGGTGAGGAAAGGGACAATGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CAACTGGGTGAGGAAAGGGAC
Primer_r	TGCTTCTTGCCAGGTGGGAGG
PCR product length	159 bp
PCR conditions	95 °C15 sec68 °C60 sec30 cycles