##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BbrPI       BlnI        BsiWI       BsmBI       
BsmI        BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     CpoI        DrdI        Eam1105I    EcoRI       
EcoRV       HindIII     KpnI        MfeI        MunI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SapI        SfuI        SgrAI       SnaBI       
SpeI        XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccIII        348  TCCGGA               T^CCGGA
AflII        4830  CTTAAG               C^TTAAG
Aor51HI      4822  AGCGCT               AGC^GCT
AviII        1324  TGCGCA               TGC^GCA
BclI         2047  TGATCA               T^GATCA
BfrI         4830  CTTAAG               C^TTAAG
BsaBI        1402  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         348  TCCGGA               T^CCGGA
BspDI        2030  ATCGAT               AT^CGAT
BspEI         348  TCCGGA               T^CCGGA
BspHI        1338  TCATGA               T^CATGA
ClaI         2030  ATCGAT               AT^CGAT
DraI         5848  TTTAAA               TTT^AAA
DraIII       3546  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     4822  AGCGCT               AGC^GCT
EcoT22I      5423  ATGCAT               ATGCA^T
FbaI         2047  TGATCA               T^GATCA
FspI         1324  TGCGCA               TGC^GCA
HpaI         3480  GTTAAC               GTT^AAC
MamI         1402  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1752  ACGCGT               A^CGCGT
MroI          348  TCCGGA               T^CCGGA
NsiI         5423  ATGCAT               ATGCA^T
RcaI         1338  TCATGA               T^CATGA
SacI         1352  GAGCTC               GAGCT^C
SalI         2728  GTCGAC               G^TCGAC
SbfI         4272  CCTGCAGG             CCTGCA^GG
ScaI         2115  AGTACT               AGT^ACT
SexAI        3375  ACCAGGT              A^CC[AT]GGT
Sse8387I     4272  CCTGCAGG             CCTGCA^GG
SwaI         5847  ATTTAAAT             ATTT^AAAT
XbaI         5113  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Asp700I       520  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5291  GAAGGGTTTC          
AspI         1262  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4332  GACCCCGTC           
BamHI        2131  GGATCC               G^GATCC
             5670  GGATCC              
BcgI         1132  GCATGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1843  GCAGCGGACTCG        
BciVI         412  GGATAC               GTATCC(6/5)
             4975  GGATAC              
BglII        1242  AGATCT               A^GATCT
             1407  AGATCT              
BlpI         2714  GCTCAGC              GC^T[ACGT]AGC
             3860  GCTGAGC             
Bpu1102I     2714  GCTCAGC              GC^T[ACGT]AGC
             3860  GCTGAGC             
BsaAI         773  TACGTG               [CT]AC^GT[GA]
             1518  TACGTG              
BsaI         1094  GAGACC               GGTCTC(1/5)
             2612  GAGACC              
BsaWI         348  TCCGGA               [AT]^CCGG[AT]
             2808  ACCGGA              
Bsp1407I     3900  TGTACA               T^GTACA
             3912  TGTACA              
BspLU11I      135  ACATGT               A^CATGT
             2415  ACATGT              
BsrGI        3900  TGTACA               T^GTACA
             3912  TGTACA              
BstEII       3358  GGTGACC              G^GT[ACGT]ACC
             4457  GGTGACC             
BstPI        3358  GGTGACC              G^GT[ACGT]ACC
             4457  GGTGACC             
CelII        2714  GCTCAGC              GC^T[ACGT]AGC
             3860  GCTGAGC             
EcoNI        3370  CCTGTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4663  CCTACTTCAGG         
EcoO65I      3358  GGTGACC              G^GT[ACGT]ACC
             4457  GGTGACC             
HincII       2728  GTCGAC               GT[CT]^[GA]AC
             3480  GTTAAC              
HindII       2728  GTCGAC               GT[CT]^[GA]AC
             3480  GTTAAC              
PflFI        1262  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4332  GACCCCGTC           
SfiI         1661  GGCCGGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1825  GGCCATGCTGGCC       
SmaI         1452  CCCGGG               CCC^GGG
             2862  CCCGGG              
SphI          903  GCATGC               GCATG^C
             2710  GCATGC              
SspBI        3900  TGTACA               T^GTACA
             3912  TGTACA              
Tth111I      1262  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4332  GACCCCGTC           
XmaCI        1452  CCCGGG               C^CCGGG
             2862  CCCGGG              
XmaI         1452  CCCGGG               C^CCGGG
             2862  CCCGGG              
XmnI          520  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5291  GAAGGGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  144 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       AocI        ApaI        ApaLI       ApoI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FseI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII