Gene/Protein Characteristic Table for KIAA0093
Order Kazusa clone(s) from : Japan || Other countries
Product ID ORK00406
Accession No D42055
Description neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase, transcript variant 1
Clone name ha00935
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (5749 bp)
Predicted protein sequence (927 aa)
Flexi ORF Clone FXC00406
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0093 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5749 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2964 bp
Genome contig ID gi51511731r_53806423
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
TCTTGAATCCACAAATAAAGTTCTATTCTGATTTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAAAACAAAACAAAACTGTGGTTCATTATAAAATAGGAGTGTTTCCAAG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 15 r 53906423 54073127 29 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 927 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
P46934 0 100.0 E3 ubiquitin-pr...
Homo sapiens
XP_001088005 0 98.8 neural precurso...
Macaca mulatta
EAW77495 0 100.0 neural precurso...
Homo sapiens
EAW77496 0 99.9 neural precurso...
Homo sapiens
AAT52215 0 99.9 cell proliferat...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB007899 1.1e-101 65.6 KIAA0439
AB037722 1.3e-46 36.7 KIAA1301
AB002320 3.9e-46 36.2 KIAA0322
AB046845 6.4e-46 45.4 KIAA1625
AB002310 8.5e-39 48.7 KIAA0312
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR000008 63 75 PR00360 C2 calcium-dependent membrane targeting
IPR000008 93 106 PR00360 C2 calcium-dependent membrane targeting
HMMPfam IPR000008 48 134 PF00168 C2 calcium-dependent membrane targeting
IPR001202 220 249 PF00397 WW/Rsp5/WWP
IPR001202 377 406 PF00397 WW/Rsp5/WWP
IPR001202 450 479 PF00397 WW/Rsp5/WWP
IPR001202 502 531 PF00397 WW/Rsp5/WWP
IPR000569 621 926 PF00632 HECT
HMMSmart IPR000008 47 149 SM00239 C2 calcium-dependent membrane targeting
IPR001202 219 251 SM00456 WW/Rsp5/WWP
IPR001202 376 408 SM00456 WW/Rsp5/WWP
IPR001202 449 481 SM00456 WW/Rsp5/WWP
IPR001202 501 533 SM00456 WW/Rsp5/WWP
IPR000569 590 926 SM00119 HECT
ProfileScan IPR000008 48 134 PS50004 C2 calcium-dependent membrane targeting
IPR001202 218 251 PS50020 WW/Rsp5/WWP
IPR001202 375 408 PS50020 WW/Rsp5/WWP
IPR001202 448 481 PS50020 WW/Rsp5/WWP
IPR001202 500 533 PS50020 WW/Rsp5/WWP
IPR000569 592 926 PS50237 HECT
ScanRegExp IPR001202 224 249 PS01159 WW/Rsp5/WWP
IPR001202 381 406 PS01159 WW/Rsp5/WWP
IPR001202 454 479 PS01159 WW/Rsp5/WWP
IPR001202 506 531 PS01159 WW/Rsp5/WWP
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 15
Experimental conditions
Panel name Genebridge 4
Primer_f TGTGCTATGCTCTGGGGTAAG
Primer_r TGCTGATGCTGTGGTGTTTGG
PCR product length 491 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
Order Kazusa clone(s) from : Japan || Other countries
Back to the HUGE Protein Database homepage
Send a message to office AT kazusa.or.jp