Gene/Protein Characteristic Table for KIAA1225
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Product ID ORK00772
Accession No AB033051
Description erbb2 interacting protein, transcript variant 2
Clone name fh04221s1
Vector information
The cDNA fragment was originally inserted at SalI-NotI site ...
cDNA sequence DNA sequence (6064 bp)
Predicted protein sequence (1371 aa)
Flexi ORF Clone FXC00772
Source Human fetal brain
Rouge ID mKIAA1225 by Kazusa Mouse cDNA Project
Note We replaced fh04221, former representative clones for KIAA1225 with fh04221s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 6064 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1948 bp
Genome contig ID gi51511721f_65224303
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
GATAAAATTCACTTATTGTATGTGTGTTGTAATCT
Flanking genome sequence
(187761 - 187810)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAGAATGACAAACAGCTTCTTTAAGACAAGTCTCGGTGTTC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 5 f 65324303 65412062 23 99.9 Perfect prediction
Features of the protein sequence
Description

Length: 1371 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAI26465 0 99.9 Erbb2 interacti...
Homo sapiens
XP_001162804 0 99.8 ERBB2 interacti...
Pan troglodytes
AAI15013 0 99.6 Erbb2 interacti...
Homo sapiens
AAI15016 0 99.1 ERBB2IP protein...
Homo sapiens
BAG65463 0 98.0 unnamed protein...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D63481 1.7e-40 39.1 KIAA0147
AB020669 7.9e-13 29.7 KIAA0862
AB037786 3.4e-11 32.1 KIAA1365
AB023233 7.3e-06 22.7 KIAA1016
AB037858 8.7e-06 28.0 KIAA1437
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001611 232 245 PR00019 Leucine-rich repeat
IPR001611 367 380 PR00019 Leucine-rich repeat
HMMPfam IPR001611 47 68 PF00560 Leucine-rich repeat
IPR001611 70 91 PF00560 Leucine-rich repeat
IPR001611 93 114 PF00560 Leucine-rich repeat
IPR001611 139 160 PF00560 Leucine-rich repeat
IPR001611 162 183 PF00560 Leucine-rich repeat
IPR001611 185 206 PF00560 Leucine-rich repeat
IPR001611 231 252 PF00560 Leucine-rich repeat
IPR001611 254 275 PF00560 Leucine-rich repeat
IPR001611 277 298 PF00560 Leucine-rich repeat
IPR001611 300 321 PF00560 Leucine-rich repeat
IPR001611 346 367 PF00560 Leucine-rich repeat
IPR001611 369 390 PF00560 Leucine-rich repeat
IPR001478 1282 1366 PF00595 PDZ/DHR/GLGF
HMMSmart NULL 45 63 SM00365 NULL
NULL 45 64 SM00364 NULL
IPR003591 48 68 SM00369 Leucine-rich repeat
NULL 68 87 SM00364 NULL
NULL 91 113 SM00365 NULL
NULL 91 110 SM00364 NULL
IPR003591 91 114 SM00369 Leucine-rich repeat
IPR003591 137 159 SM00369 Leucine-rich repeat
NULL 160 191 SM00365 NULL
IPR003591 160 182 SM00369 Leucine-rich repeat
NULL 160 179 SM00364 NULL
NULL 183 202 SM00364 NULL
IPR003591 183 205 SM00369 Leucine-rich repeat
NULL 206 227 SM00365 NULL
IPR003591 206 228 SM00369 Leucine-rich repeat
IPR003591 229 252 SM00369 Leucine-rich repeat
NULL 229 251 SM00365 NULL
NULL 252 271 SM00364 NULL
IPR003591 253 274 SM00369 Leucine-rich repeat
IPR003591 275 298 SM00369 Leucine-rich repeat
NULL 275 294 SM00364 NULL
IPR003591 321 344 SM00369 Leucine-rich repeat
NULL 345 363 SM00364 NULL
IPR003591 345 366 SM00369 Leucine-rich repeat
NULL 367 386 SM00364 NULL
NULL 367 385 SM00365 NULL
IPR003591 367 389 SM00369 Leucine-rich repeat
NULL 390 411 SM00365 NULL
IPR001478 1289 1369 SM00228 PDZ/DHR/GLGF
ProfileScan IPR001478 1280 1369 PS50106 PDZ/DHR/GLGF
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TTCGAGCTAATACTGCATACC
Primer_r TCACCAAGATTTACAGAGGCT
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 5
Experimental conditions
Panel name unigene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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