Gene/Protein Characteristic Table for KIAA1512
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Product ID ORK06072
Accession No AB040945
Description myosin, heavy chain 7B, cardiac muscle, beta
Clone name fg04660y1
Vector information
The cDNA fragment was originally inserted at BssH II-NotI si ...
cDNA sequence DNA sequence (6289 bp)
Predicted protein sequence (2010 aa)
Flexi ORF Clone FXC06072
Source Human fetal brain
Note We replaced fg04660, former representative clones for KIAA1512 with fg04660y1. (2002/12/27)
Features of the cloned cDNA sequence
Description

Length: 6289 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 245 bp
Genome contig ID gi51511747f_32929096
PolyA signal sequence
(AATAAA,-20)
+----*----+----*----+----*----+----
AGCAGTCATTTTTAAAATAAAGTTATTTAATAGTC
Flanking genome sequence
(124803 - 124852)
----+----*----+----*----+----*----+----*----+----*
TCCATTTAATTGGTTTATTTGCTGTTAATAAATTGGCAACACAAGGAAGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 20 f 33026867 33053897 43 99.8 Perfect prediction
Features of the protein sequence
Description

Length: 2010 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAI51243 0 100.0 Myosin, heavy c...
Homo sapiens
CAI19310 0 100.0 myosin, heavy c...
Homo sapiens
XP_001160839 0 99.9 myosin, heavy p...
Pan troglodytes
A7E2Y1 0 100.0 Myosin-7B; Myos...
Homo sapiens
XP_001160798 0 99.7 myosin, heavy p...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB023217 1.4e-155 60.6 KIAA1000
AB020673 1.7e-104 40.4 KIAA0866
AB111886 4.5e-53 36.8 KIAA2034
AB032945 4e-34 30.4 KIAA1119
AB018270 6.4e-25 32.1 KIAA0727
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR001609 289 319 PD000355 Myosin head
FPrintScan IPR001609 183 202 PR00193 Myosin head
IPR001609 239 264 PR00193 Myosin head
IPR001609 297 324 PR00193 Myosin head
IPR001609 528 556 PR00193 Myosin head
IPR001609 582 610 PR00193 Myosin head
HMMPfam IPR004009 101 143 PF02736 Myosin
IPR001609 155 842 PF00063 Myosin head
IPR000048 858 878 PF00612 IQ calmodulin-binding region
IPR002928 1144 2002 PF01576 Myosin tail
HMMSmart IPR001609 147 855 SM00242 Myosin head
ProfileScan IPR000048 857 886 PS50096 IQ calmodulin-binding region
ScanRegExp IPR000169 404 414 PS00639 Peptidase
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CCACGAGGAGGCACTTGAAGC
Primer_r AGCCTGGATCTCACTCTTCTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 20
Experimental conditions
Panel name GeneBridge 4
Primer_f GCCTCAACCTTCTGCATTCGC
Primer_r GTCTTCTTCATCCGTTCCAGG
PCR product length 220(370) bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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