Gene/Protein Characteristic Table for KIAA2000
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Product ID ORK06179
Accession No AB082531
Description nephronophthisis 3 (adolescent)
Clone name bf00135
Vector information
The cDNA fragment was inserted at the XhoI-SacI site of the ...
cDNA sequence DNA sequence (7784 bp)
Predicted protein sequence (699 aa)
Source Human adult brain
Rouge ID mKIAA2000 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 7784 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning Warning
Integrity of 3' end
Length of 3'UTR 3704 bp
Genome contig ID gi89161205r_133659676
PolyA signal sequence
(ATTAAA,-23)
+----*----+----*----+----*----+----
TGAACATTAGGAATTAAAGGCTATATCTGGTCCTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACAATTTTGTAATTTGTTTCTCTGTCTGAGGAAAGCTGGCTGGATTGGTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 3 r 133759676 133923993 45 99.7 Perfect prediction
Features of the protein sequence
Description

Length: 699 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW79187 0 99.9 nephronophthisi...
Homo sapiens
Q7Z494 0 99.9 Nephrocystin-3.
Homo sapiens
XP_516758 0 99.0 nephronophthisi...
Pan troglodytes
XP_001115440 0 97.9 similar to neph...
Macaca mulatta
XP_001495737 0 94.7 nephronophthisi...
Equus caballus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB014564 5.7e-08 23.8 KIAA0664
AB058688 5.6e-05 26.4 KIAA1785
AB028966 5.8e-05 24.2 KIAA1043
D86981 0.0003 25.2 KIAA0228
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR002151 428 447 PR00381 Kinesin light chain
IPR002151 450 468 PR00381 Kinesin light chain
IPR002151 515 532 PR00381 Kinesin light chain
IPR002151 559 579 PR00381 Kinesin light chain
IPR002151 594 615 PR00381 Kinesin light chain
HMMPfam IPR001440 285 318 PF00515 Tetratricopeptide TPR_1
IPR001440 327 360 PF00515 Tetratricopeptide TPR_1
IPR001440 369 402 PF00515 Tetratricopeptide TPR_1
IPR001440 435 468 PF00515 Tetratricopeptide TPR_1
IPR001440 477 510 PF00515 Tetratricopeptide TPR_1
IPR001440 519 552 PF00515 Tetratricopeptide TPR_1
IPR001440 561 594 PF00515 Tetratricopeptide TPR_1
IPR013105 603 636 PF07719 Tetratricopeptide TPR_2
HMMSmart IPR013026 285 318 SM00028 Tetratricopeptide region
IPR013026 327 360 SM00028 Tetratricopeptide region
IPR013026 369 402 SM00028 Tetratricopeptide region
IPR013026 435 468 SM00028 Tetratricopeptide region
IPR013026 477 510 SM00028 Tetratricopeptide region
IPR013026 519 552 SM00028 Tetratricopeptide region
IPR013026 561 594 SM00028 Tetratricopeptide region
IPR013026 603 636 SM00028 Tetratricopeptide region
ProfileScan IPR013026 285 318 PS50005 Tetratricopeptide region
IPR013026 285 636 PS50293 Tetratricopeptide region
IPR013026 327 360 PS50005 Tetratricopeptide region
IPR013026 369 402 PS50005 Tetratricopeptide region
IPR013026 435 468 PS50005 Tetratricopeptide region
IPR013026 477 510 PS50005 Tetratricopeptide region
IPR013026 519 552 PS50005 Tetratricopeptide region
IPR013026 561 594 PS50005 Tetratricopeptide region
IPR013026 603 636 PS50005 Tetratricopeptide region
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CTAAACAAGACCAGCAAAGCC
Primer_r CTGCCCAAATCCTTAGTCTCC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 3
Experimental conditions
Panel name genbank
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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