##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
AocI        AscI        AseI        AsnI        AspEI       
AvrII       BciVI       BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BsiWI       BspDI       Bst1107I    
BstZ17I     Bsu36I      CelII       ClaI        DraI        
Eam1105I    Eco81I      EcoRV       EcoT22I     FseI        
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        SacII       
SalI        SapI        SfiI        SgrAI       SnaBI       
SpeI        SspI        SwaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Aor51HI      1863  AGCGCT               AGC^GCT
Asp700I      1816  GAAAGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1278  TGCGCA               TGC^GCA
BamHI        1557  GGATCC               G^GATCC
BbvCI        1096  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1022  CGAGGCCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1766  CTAG                 C^TAG
BglII        2028  AGATCT               A^GATCT
BsmI         2209  GAATGC               GAATGC(1/-1)
BspHI        2229  TCATGA               T^CATGA
BstAPI        844  GCAGCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         512  TTCGAA               TT^CGAA
CpoI         1244  CGGACCG              CG^G[AT]CCG
DraIII       2219  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1669  GACCCCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1863  AGCGCT               AGC^GCT
EcoRI         515  GAATTC               G^AATTC
FspI         1278  TGCGCA               TGC^GCA
MaeI         1766  CTAG                 C^TAG
MseI         1836  TTAA                 T^TAA
NcoI         3318  CCATGG               C^CATGG
NspV          512  TTCGAA               TT^CGAA
PaeR7I       2819  CTCGAG               C^TCGAG
RcaI         2229  TCATGA               T^CATGA
RsrII        1244  CGGACCG              CG^G[AT]CCG
SacI          458  GAGCTC               GAGCT^C
SbfI         2452  CCTGCAGG             CCTGCA^GG
ScaI          780  AGTACT               AGT^ACT
SexAI         993  ACCTGGT              A^CC[AT]GGT
SfuI          512  TTCGAA               TT^CGAA
Sse8387I     2452  CCTGCAGG             CCTGCA^GG
StuI          828  AGGCCT               AGG^CCT
Tru9I        1836  TTAA                 T^TAA
XbaI         1765  TCTAGA               T^CTAGA
XhoI         2819  CTCGAG               C^TCGAG
XmnI         1816  GAAAGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I       1394  GGTACC               G^GTACC
             3626  GGTACC              
AccIII        870  TCCGGA               T^CCGGA
             1554  TCCGGA              
AcyI          186  GGCGCC               G[GA]^CG[CT]C
             2606  GGCGCC              
AflIII        858  ACATGT               A^C[GA][CT]GT
             1533  ACATGT              
Asp718I      1394  GGTACC               G^GTACC
             3626  GGTACC              
AspI          959  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3560  GACCGCGTC           
BbeI          186  GGCGCC               GGCGC^C
             2606  GGCGCC              
BbrPI        2264  CACGTG               CAC^GTG
             2912  CACGTG              
BclI         2232  TGATCA               T^GATCA
             2916  TGATCA              
BsaHI         186  GGCGCC               G[GA]^CG[CT]C
             2606  GGCGCC              
BseAI         870  TCCGGA               T^CCGGA
             1554  TCCGGA              
BsmBI         964  CGTCTC               CGTCTC(1/5)
             1653  GAGACG              
BspEI         870  TCCGGA               T^CCGGA
             1554  TCCGGA              
BspLU11I      858  ACATGT               A^CATGT
             1533  ACATGT              
EcoNI        2781  CCTCCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2797  CCTGAGCCAGG         
FbaI         2232  TGATCA               T^GATCA
             2916  TGATCA              
Hin1I         186  GGCGCC               G[GA]^CG[CT]C
             2606  GGCGCC              
KasI          186  GGCGCC               G^GCGCC
             2606  GGCGCC              
KpnI         1394  GGTACC               GGTAC^C
             3626  GGTACC              
MroI          870  TCCGGA               T^CCGGA
             1554  TCCGGA              
NarI          186  GGCGCC               GG^CGCC
             2606  GGCGCC              
PflFI         959  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3560  GACCGCGTC           
PflMI        2218  CCACCGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3759  CCATCCGCTGG         
PmaCI        2264  CACGTG               CAC^GTG
             2912  CACGTG              
PmlI         2264  CACGTG               CAC^GTG
             2912  CACGTG              
SfoI          186  GGCGCC               GGC^GCC
             2606  GGCGCC              
SphI         2258  GCATGC               GCATG^C
             2617  GCATGC              
TfiI         1130  GATTC                G^A[AT]TC
             1251  GATTC               
Tth111I       959  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3560  GACCGCGTC           
Van91I       2218  CCACCGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3759  CCATCCGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BglI        BmyI        BpmI        BpuAI       
BsaAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SspBI       StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmaCI       XmaI