KIAA1512

Product ID	ORK06072
Accession No	AB040945
Description	myosin, heavy chain 7B, cardiac muscle, beta
Clone name	fg04660y1
Vector information	The cDNA fragment was originally inserted at BssH II-NotI si ... The cDNA fragment was originally inserted at BssH II-NotI site of the pBluescript II SK(+) vector (GenBank Accession No.X52328, AmpR). The adjacent sequence to the 5' end of the cDNA is as follows: GCGCGCTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAGGTCGAC CCACGCGTCC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC CCCCTCGAGG TCGACCCACG TAATACGACT CACTATAGGG CGAATTGGGT ACCGGGCCCC CCCTCGAGGT CGACCCACGC GTCCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC GAGGTCGATC CACGCGTCCG-5' end of cDNA.
cDNA sequence	DNA sequence (6289 bp) Predicted protein sequence (2010 aa)
HaloTag ORF Clone	FHC06072
Flexi ORF Clone	FXC06072
Source	Human fetal brain
Note	We replaced fg04660, former representative clones for KIAA1512 with fg04660y1. (2002/12/27)

Length: 6289 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	245 bp
Genome contig ID	gi51511747f_32929096
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- AGCAGTCATTTTTAAAATAAAGTTATTTAATAGTC
Flanking genome sequence (124803 - 124852)	----+--------+--------+--------+--------+----* TCCATTTAATTGGTTTATTTGCTGTTAATAAATTGGCAACACAAGGAAGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	20	f	33026867	33053897	43	99.8	Perfect prediction

Length: 2010 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI51243	100.0	Myosin, heavy c...	Homo sapiens
CAI19310	100.0	myosin, heavy c...	Homo sapiens
XP_001160839	99.9	myosin, heavy p...	Pan troglodytes
A7E2Y1	100.0	Myosin-7B; Myos...	Homo sapiens
XP_001160798	99.7	myosin, heavy p...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB023217	1.4e-155	60.6	KIAA1000
AB020673	1.7e-104	40.4	KIAA0866
AB111886	4.5e-53	36.8	KIAA2034
AB032945	4e-34	30.4	KIAA1119
AB018270	6.4e-25	32.1	KIAA0727

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001609	289	319	PD000355	Myosin head
FPrintScan	IPR001609	183	202	PR00193	Myosin head
	IPR001609	239	264	PR00193	Myosin head
	IPR001609	297	324	PR00193	Myosin head
	IPR001609	528	556	PR00193	Myosin head
	IPR001609	582	610	PR00193	Myosin head
HMMPfam	IPR004009	101	143	PF02736	Myosin
	IPR001609	155	842	PF00063	Myosin head
	IPR000048	858	878	PF00612	IQ calmodulin-binding region
	IPR002928	1144	2002	PF01576	Myosin tail
HMMSmart	IPR001609	147	855	SM00242	Myosin head
ProfileScan	IPR000048	857	886	PS50096	IQ calmodulin-binding region
ScanRegExp	IPR000169	404	414	PS00639	Peptidase

Primer_f	CCACGAGGAGGCACTTGAAGC
Primer_r	AGCCTGGATCTCACTCTTCTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GCCTCAACCTTCTGCATTCGC
Primer_r	GTCTTCTTCATCCGTTCCAGG
PCR product length	220(370) bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles