Gene/Protein Characteristic Table for KIAA1477
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Product ID ORK00236
Accession No AB040910
Description MAP/microtubule affinity-regulating kinase 1, transcript variant 4
Clone name fj04927
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4638 bp)
Predicted protein sequence (870 aa)
Flexi ORF Clone FXC00236
Source Human fetal brain
Rouge ID mKIAA1477 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4638 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1764 bp
Genome contig ID gi89161185f_218668191
PolyA signal sequence
(AATAAA,-26)
+----*----+----*----+----*----+----
AGTTCTCATAATAAAGCACTTGTCTTTTGCTCTTT
Flanking genome sequence
(235706 - 235755)
----+----*----+----*----+----*----+----*----+----*
ATCAGAATGTGAATTACCTGTTTTCTGGTACAAAAGTATTCTGTATGAGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 f 218768191 218903895 16 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 870 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAG11330 1.9e-209 100.0 serine/threonin...
synthetic construct
CAH72462 4.8e-209 99.9 MAP/microtubule...
Homo sapiens
EAW93298 1.9e-176 97.1 MAP/microtubule...
Homo sapiens
XP_525067 2.1e-175 96.5 MAP/microtubule...
Pan troglodytes
XP_001172814 7.9e-173 95.8 MAP/microtubule...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB058763 4.5e-34 54.9 KIAA1860
AB023216 4.9e-18 51.7 KIAA0999
AB018324 5.2e-17 50.6 KIAA0781
AB058714 7.4e-13 32.3 KIAA1811
AB011109 8e-12 29.6 KIAA0537
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR000719 172 401 PD000001 Protein kinase
HMMPfam IPR000719 172 401 PF00069 Protein kinase
IPR000449 421 460 PF00627 Ubiquitin-associated/Translation elongation factor EF1B
IPR001772 821 870 PF02149 Kinase-associated KA1
HMMSmart IPR001245 172 400 SM00219 Tyrosine protein kinase
IPR002290 172 401 SM00220 Serine/threonine protein kinase
IPR000449 422 459 SM00165 Ubiquitin-associated/Translation elongation factor EF1B
ProfileScan IPR000719 172 401 PS50011 Protein kinase
IPR000449 415 460 PS50030 Ubiquitin-associated/Translation elongation factor EF1B
IPR001772 821 870 PS50032 Kinase-associated KA1
ScanRegExp IPR000719 178 201 PS00107 Protein kinase
IPR008271 268 280 PS00108 Serine/threonine protein kinase
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AAAGACCTCAGGCTAACAGTG
Primer_r TATTCCTTCTTGCCATGCTAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name GeneBridge 4
Primer_f CGAGCCAGGCAGTGATTTGAG
Primer_r GGTATGCGGACAGTAACGAGG
PCR product length 178 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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