Gene/Protein Characteristic Table for KIAA0999
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Product ID ORK06551
Accession No AB023216
Description SIK family kinase 3
Clone name fj10213
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4460 bp)
Predicted protein sequence (1371 aa)
Source Human fetal brain
Rouge ID mKIAA0999 by Kazusa Mouse cDNA Project
Note We replaced hk08707, former representative clones for KIAA0999 with fj10213. (2001/5/29)
Features of the cloned cDNA sequence
Description

Length: 4460 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Features of the protein sequence
Description

Length: 1371 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_582999 0 93.0 similar to Seri...
Bos taurus
XP_001500846 0 95.4 similar to Seri...
Equus caballus
EDL25676 1e-162 89.7 cDNA sequence B...
Mus musculus
AAH82313 8.8e-155 87.3 BC033915 protei...
Mus musculus
NP_081774 2.7e-152 87.8 serine/threonin...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB018324 6.3e-40 36.7 KIAA0781
AB058763 7.8e-31 52.2 KIAA1860
D43636 1.4e-21 38.0 KIAA0096
AB040910 2.7e-21 51.7 KIAA1477
AB011109 5.9e-21 37.5 KIAA0537
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR000719 116 367 PD000001 Protein kinase
HMMPfam IPR000719 116 367 PF00069 Protein kinase
HMMSmart IPR001245 116 367 SM00219 Tyrosine protein kinase
IPR002290 116 367 SM00220 Serine/threonine protein kinase
ProfileScan IPR000719 116 367 PS50011 Protein kinase
IPR000449 394 434 PS50030 Ubiquitin-associated/Translation elongation factor EF1B
ScanRegExp IPR000719 122 145 PS00107 Protein kinase
IPR008271 234 246 PS00108 Serine/threonine protein kinase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 7 VGTGARASAVAAVPSVSLLSGAS 29 SECONDARY 23
2 31 SSLAWCGLATGALGAGARGIPEL 53 SECONDARY 23
3 295 DIWSLGVVLYVLVCGALPFDGST 317 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TCACCAACTACTCACCAGAAG
Primer_r CAAGATGGCACGGTTAGTAGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name GeneBridge 4
Primer_f TGACTTATCTGCGCTTGTTCC
Primer_r CAAGATGGCACGGTTAGTAGG
PCR product length 164 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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