Gene/Protein Characteristic Table for KIAA1424
Order Kazusa clone(s) from : Japan || Other countries
Product ID ORK04154
Accession No AB037845
Description Rho GTPase activating protein 21
Clone name ha06448
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6631 bp)
Predicted protein sequence (1944 aa)
Source Human adult brain
Rouge ID mKIAA1424 by Kazusa Mouse cDNA Project
Note We replaced hh15972, former representative clones for KIAA1424 with ha06448. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 6631 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 794 bp
Genome contig ID gi89161187r_24812551
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
CCAATTAAACACTGGAAATAAAAATTGTTTTGGTG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AATTTTGCATGGCTTGAAACTTATTCTGATATATTTTTAGATGTCATCCA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 10 r 24912551 25050794 25 99.6 Perfect prediction
Ensembl gnome browser 6 r 80829934 80836598 2 97.0 Perfect prediction
Features of the protein sequence
Description

Length: 1944 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q5T5U3 0 100.0 Rho GTPase-acti...
Homo sapiens
NP_065875 0 100.0 Rho GTPase acti...
Homo sapiens
AAM22955 0 99.7 Rho-GTPase acti...
Homo sapiens
EAW86122 0 99.7 Rho GTPase acti...
Homo sapiens
XP_507699 0 99.5 Rho GTPase acti...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB040934 2.7e-41 48.6 KIAA1501
AB037725 1.1e-09 22.9 KIAA1304
AB007871 1.4e-08 30.6 KIAA0411
AB051509 1.4e-08 28.0 KIAA1722
AB014572 4.1e-08 29.2 KIAA0672
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001478 36 142 PF00595 PDZ/DHR/GLGF
IPR001849 918 1026 PF00169 Pleckstrin-like
IPR000198 1148 1301 PF00620 RhoGAP
HMMSmart IPR001478 44 145 SM00228 PDZ/DHR/GLGF
IPR001849 918 1028 SM00233 Pleckstrin-like
IPR000198 1145 1322 SM00324 RhoGAP
ProfileScan IPR001478 36 145 PS50106 PDZ/DHR/GLGF
IPR001849 917 1026 PS50003 Pleckstrin-like
IPR000198 1133 1325 PS50238 RhoGAP
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f ACAGTTGGTTTTTAGGGTGCC
Primer_r ACCTGGAATGACTAAAGAATG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 6
Experimental conditions
Panel name GeneBridge 4
Primer_f ACAGTTGGTTTTTAGGGTGCC
Primer_r ACCTGGAATGACTAAAGAATG
PCR product length 150 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
Order Kazusa clone(s) from : Japan || Other countries
Back to the HUGE Protein Database homepage
Send a message to office AT kazusa.or.jp