Gene/Protein Characteristic Table for KIAA1140
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Product ID ORK00184
Accession No AB032966
Description tetratricopeptide repeat domain 7A
Clone name hk01004s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4748 bp)
Predicted protein sequence (752 aa)
Flexi ORF Clone FXC00184
Source Human adult brain
Rouge ID mKIAA1140 by Kazusa Mouse cDNA Project
Note We replaced hk01004, former representative clones for KIAA1140 with hk01004s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 4748 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning Warning
Integrity of 3' end
Length of 3'UTR 2223 bp
Genome contig ID gi89161199f_46931064
PolyA signal sequence
(ATTAAA,-34)
+----*----+----*----+----*----+----
CATTAAAACAACTCTAAAGAACGCTGCTCATTTAC
Flanking genome sequence
(225718 - 225767)
----+----*----+----*----+----*----+----*----+----*
ACAGAAGCTCTGTTTCTTCCTGTACCCCAGGGGGTCACCATAGCACCCCC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 f 47031064 47156780 21 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 752 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAG10426 0 100.0 tetratricopepti...
synthetic construct
EAX00228 0 99.9 tetratricopepti...
Homo sapiens
EAX00227 0 99.9 tetratricopepti...
Homo sapiens
EAX00226 0 99.9 tetratricopepti...
Homo sapiens
Q9ULT0 0 99.9 Tetratricopepti...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001440 460 493 PF00515 Tetratricopeptide TPR_1
IPR013105 639 672 PF07719 Tetratricopeptide TPR_2
IPR001440 707 740 PF00515 Tetratricopeptide TPR_1
HMMSmart IPR013026 308 341 SM00028 Tetratricopeptide region
IPR013026 381 425 SM00028 Tetratricopeptide region
IPR013026 460 493 SM00028 Tetratricopeptide region
IPR013026 605 638 SM00028 Tetratricopeptide region
IPR013026 639 672 SM00028 Tetratricopeptide region
IPR013026 673 706 SM00028 Tetratricopeptide region
IPR013026 707 740 SM00028 Tetratricopeptide region
ProfileScan IPR013026 274 341 PS50293 Tetratricopeptide region
IPR013026 308 341 PS50005 Tetratricopeptide region
IPR013026 410 493 PS50293 Tetratricopeptide region
IPR013026 426 459 PS50005 Tetratricopeptide region
IPR013026 460 493 PS50005 Tetratricopeptide region
IPR013026 605 638 PS50005 Tetratricopeptide region
IPR013026 605 740 PS50293 Tetratricopeptide region
IPR013026 639 672 PS50005 Tetratricopeptide region
IPR013026 673 706 PS50005 Tetratricopeptide region
IPR013026 707 740 PS50005 Tetratricopeptide region
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f AGAAGTCCATACCAGTACACC
Primer_r AATGAGCAGCGTTCTTTAGAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name GeneBridge 4
Primer_f AGAAGTCCATACCAGTACACC
Primer_r AATGAGCAGCGTTCTTTAGAG
PCR product length 101 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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