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| Product ID | ORK04592 | 
|---|---|
| Accession No | AB020709 | 
| Description | connector enhancer of kinase suppressor of Ras 2 | 
| Clone name | hk09777 | 
| Vector information | |
| cDNA sequence | DNA sequence (4349 bp) Predicted protein sequence (910 aa) | 
| Source | Human adult brain | 
| Rouge ID | mKIAA0902
    
    by Kazusa Mouse cDNA Project | 
 Length: 4349 bp
 Length: 4349 bp Physical map
 Physical map 
     Restriction map
 Restriction map Prediction of protein coding region (GeneMark analysis).
 Prediction of protein coding region (GeneMark analysis).
    | N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | Warning | 
 Integrity of 3' end
 Integrity of 3' end
    | Length of 3'UTR | 1211 bp | 
|---|---|
| Genome contig ID | gi89161218f_21202481 | 
| PolyA signal sequence (AATAAA,-27) | +----*----+----*----+----*----+---- | 
| Flanking genome sequence (368294 - 368343) | ----+----*----+----*----+----*----+----*----+----* | 
 Length: 910 aa
 
        Length: 910 aa Result of homology search against nr database 
        (FASTA output,
	Multiple alignment)
        Result of homology search against nr database 
        (FASTA output,
	Multiple alignment)|  | 
 | 
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
 Result of homology search against HUGE database
        (FASTA output,
	Multiple alignment)
        Result of homology search against HUGE database
        (FASTA output,
	Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
 Result of motif / domain search (InterProScan and SOSUI)
	Result of motif / domain search (InterProScan and SOSUI) Result of InterProScan
 Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition | 
|---|---|---|---|---|---|
| HMMPfam | IPR001660 | 26 | 90 | PF00536 | Sterile alpha motif SAM | 
| IPR001478 | 234 | 311 | PF00595 | PDZ/DHR/GLGF | |
| IPR010599 | 349 | 532 | PF06663 | Connector enhancer of kinase suppressor of ras 2 | |
| IPR001849 | 588 | 686 | PF00169 | Pleckstrin-like | |
| HMMSmart | IPR001660 | 25 | 93 | SM00454 | Sterile alpha motif SAM | 
| IPR001478 | 242 | 314 | SM00228 | PDZ/DHR/GLGF | |
| IPR001849 | 588 | 688 | SM00233 | Pleckstrin-like | |
| ProfileScan | IPR001660 | 28 | 93 | PS50105 | Sterile alpha motif SAM | 
| IPR001478 | 232 | 314 | PS50106 | PDZ/DHR/GLGF | |
| IPR001849 | 587 | 686 | PS50003 | Pleckstrin-like | 
|  RT-PCR-ELISA | 
 
 Experimental conditions
 Experimental conditions| Primer_f | AATCTATAGGCTGTGGGTTTC | 
|---|---|
| Primer_r | ATTGATTGAGCCATAGGGGAC | 
| PCR conditions | 95 °C  30 sec  55 °C  30 sec  72 °C  60 sec  30 cycles  | 
 Chromosome No. 1
 Chromosome No. 1 Experimental conditions
 Experimental conditions| Panel name | GeneBridge 4 | 
|---|---|
| Primer_f | AATCTATAGGCTGTGGGTTTC | 
| Primer_r | ATTGATTGAGCCATAGGGGAC | 
| PCR product length | 113 bp | 
| PCR conditions | 95 °C  15 sec  62 °C  60 sec  30 cycles | 
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