KIAA0792

Product ID	ORK00640
Accession No	AB018335
Description	transmembrane protein 63A
Clone name	hk05691
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4074 bp) Predicted protein sequence (828 aa)
HaloTag ORF Clone	FHC00640
Flexi ORF Clone	FXC00640
Source	Human adult brain
Rouge ID	mKIAA0792 by Kazusa Mouse cDNA Project

Length: 4074 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1400 bp
Genome contig ID	gi89161185r_223999863
PolyA signal sequence (AGTAAA,-19)	+--------+--------+----*----+---- TTCCAGCCGCTTTTAGAGTAAAGTCGTTTATCATC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAGCCATGTTGCTTACCACACGGAGTTGGGGGTTGGGGAGTGAGCTTGG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	224099863	224136671	25	99.6	Perfect prediction

Length: 828 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG09849	100.0	transmembrane p...	synthetic construct
O94886	99.9	Transmembrane p...	Homo sapiens
BAG51078	99.4	unnamed protein...	Homo sapiens
Q5R826	98.6	Transmembrane p...	Pongo abelii
XP_001089796	96.0	similar to tran...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB007958	6.2e-42	100.0	KIAA0489

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003864	356	790	PF02714	Protein of unknown function DUF221

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	67	TFGGIPTVLLIDVSCFLFLILVF	89	PRIMARY	23
	2	166	HIIFLLVVVSFLSLCVILPVNLS	188	PRIMARY	23
	3	212	DLLWLHTIFAVIYLFFTVGFMRH	234	PRIMARY	23
	4	446	INFTLFLGLFFLTTPSIILSTMD	468	SECONDARY	23
	5	487	FFPTLLLWSFSALLPSIVYYSTL	509	PRIMARY	23
	6	528	YIFLIFMVLILPSLGLTSLDFFF	550	PRIMARY	23
	7	583	ASAFIGNGMELLRLPGLILYTFR	605	SECONDARY	23
	8	635	MLCVFTVIVAYSITCPIIAPFGL	657	PRIMARY	23
	9	689	VNQALAAPILCLFWLYFFSFLRL	711	PRIMARY	23
	10	717	ATLFTFLVLLLTILVCLAHTCFG	739	PRIMARY	23

Primer_f	CAGCCTCTCTCACCTCTTCAG
Primer_r	ATGCTGTCCTTTGGCTCCGAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CAGCCTCTCTCACCTCTTCAG
Primer_r	ATGCTGTCCTTTGGCTCCGAG
PCR product length	144 bp
PCR conditions	95 °C15 sec68 °C60 sec30 cycles