##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccIII AclI AcsI AgeI Aor51HI
ApoI AscI AseI AsnI AviII
AvrII BbeI BcgI BglII BlnI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
FspI HpaI KasI KspI MfeI
MroI MunI NarI NdeI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tsp509I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 419 GACGTC GACGT^C
AflII 3598 CTTAAG C^TTAAG
AhdI 512 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3182 GTGCAC G^TGCAC
ApaLI 3182 GTGCAC G^TGCAC
Asp700I 1342 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 512 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1729 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 317 CTCGGG C^[CT]CG[GA]G
BamHI 2235 GGATCC G^GATCC
BbrPI 1009 CACGTG CAC^GTG
BbvCI 2416 CCTCAGC CCTCAGC(-5/-2)
BclI 2004 TGATCA T^GATCA
BfrI 3598 CTTAAG C^TTAAG
BlpI 2763 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2763 GCTGAGC GC^T[ACGT]AGC
BsaBI 534 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1836 TCCGGT [AT]^CCGG[AT]
BsmI 2743 GAATGC GAATGC(1/-1)
BsoBI 317 CTCGGG C^[CT]CG[GA]G
BspHI 1752 TCATGA T^CATGA
CelII 2763 GCTGAGC GC^T[ACGT]AGC
DraIII 2177 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 512 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2082 ATGCAT ATGCA^T
FbaI 2004 TGATCA T^GATCA
FseI 3976 GGCCGGCC GGCCGG^CC
HaeII 2633 AGCGCC [GA]GCGC^[CT]
HindIII 2864 AAGCTT A^AGCTT
MamI 534 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 999 ACGCGT A^CGCGT
NaeI 3977 GCCGGC GCC^GGC
NgoMIV 3977 GCCGGC G^CCGGC
NheI 3241 GCTAGC G^CTAGC
NsiI 2082 ATGCAT ATGCA^T
NspI 2481 ACATGC [GA]CATG^[CT]
PflFI 1729 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1009 CACGTG CAC^GTG
PmlI 1009 CACGTG CAC^GTG
PshAI 1759 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1752 TCATGA T^CATGA
SacI 1415 GAGCTC GAGCT^C
ScaI 3383 AGTACT AGT^ACT
SexAI 921 ACCTGGT A^CC[AT]GGT
SfiI 3767 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 542 TCGA T^CGA
Tth111I 1729 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 542 TCGA T^CGA
XbaI 1822 TCTAGA T^CTAGA
XmnI 1342 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 61 GGTACC G^GTACC
3483 GGTACC
AflIII 999 ACGCGT A^C[GA][CT]GT
1010 ACGTGT
ApaI 201 GGGCCC GGGCC^C
4001 GGGCCC
Asp718I 61 GGTACC G^GTACC
3483 GGTACC
BciVI 1003 GTATCC GTATCC(6/5)
3196 GTATCC
BcnI 994 CCGGG CC^[GC]GG
2953 CCGGG
BglI 2012 GCCAAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3768 GCCTGGGGGGC
BsaAI 1009 CACGTG [CT]AC^GT[GA]
2504 TACGTG
BsrDI 490 CATTGC GCAATG(2/0)
1950 GCAATG
BstEII 382 GGTCACC G^GT[ACGT]ACC
3255 GGTTACC
BstPI 382 GGTCACC G^GT[ACGT]ACC
3255 GGTTACC
BstXI 1872 CCATCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3898 CCATTGGTCTGG
EcoO65I 382 GGTCACC G^GT[ACGT]ACC
3255 GGTTACC
HincII 558 GTCAAC GT[CT]^[GA]AC
740 GTCAAC
HindII 558 GTCAAC GT[CT]^[GA]AC
740 GTCAAC
KpnI 61 GGTACC GGTAC^C
3483 GGTACC
NciI 994 CCGGG CC^[GC]GG
2953 CCGGG
SbfI 91 CCTGCAGG CCTGCA^GG
1114 CCTGCAGG
Sse8387I 91 CCTGCAGG CCTGCA^GG
1114 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI AspHI
AvaII BalI BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I TspRI
Van91I XcmI XhoII