Gene/Protein Characteristic Table for KIAA0657
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Product ID ORK06218
Accession No AB014557
Description obscurin-like 1
Clone name hk01859s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5382 bp)
Predicted protein sequence (1237 aa)
Source Human adult brain
Rouge ID mKIAA0657 by Kazusa Mouse cDNA Project
Note We replaced hk01859, former representative clones for KIAA0657 with hk01859s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 5382 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning Warning
Integrity of 3' end
Length of 3'UTR 1667 bp
Genome contig ID gi89161199r_220023695
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
TAGAGAGACAAGGAACAATAAAAGTGCTACAGCTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ATTTCCTTGCCTCTCTGAGTGATGTGGGGGGGCAGACCTTGCCTGGAGCC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 r 220123695 220143707 16 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 1237 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O75147 0 100.0 Obscurin-like p...
Homo sapiens
EAW70766 0 99.9 chondroitin pol...
Homo sapiens
ABO42328 0 92.4 obscurin-like 1...
Homo sapiens
ABO42327 0 92.3 obscurin-like 1...
Homo sapiens
ACJ76613 0 86.6 obscurin-like 1...
Oryctolagus cun...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB046776 1.3e-14 27.1 KIAA1556
D86983 2.3e-07 25.1 KIAA0230
AB046788 9.6e-07 25.1 KIAA1568
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR013098 30 62 PF07679 Immunoglobulin I-set
IPR013098 87 172 PF07679 Immunoglobulin I-set
IPR013098 177 262 PF07679 Immunoglobulin I-set
IPR003961 351 437 PF00041 Fibronectin
IPR013151 567 626 PF00047 Immunoglobulin
IPR013151 658 717 PF00047 Immunoglobulin
IPR013151 749 808 PF00047 Immunoglobulin
IPR013151 840 899 PF00047 Immunoglobulin
IPR013151 932 991 PF00047 Immunoglobulin
IPR013098 1044 1087 PF07679 Immunoglobulin I-set
IPR013151 1118 1151 PF00047 Immunoglobulin
HMMSmart IPR003599 2 63 SM00409 Immunoglobulin subtype
IPR003599 88 173 SM00409 Immunoglobulin subtype
IPR003598 94 162 SM00408 Immunoglobulin subtype 2
IPR003599 183 263 SM00409 Immunoglobulin subtype
IPR003598 189 255 SM00408 Immunoglobulin subtype 2
IPR003599 271 347 SM00409 Immunoglobulin subtype
IPR003599 458 547 SM00409 Immunoglobulin subtype
IPR003599 559 640 SM00409 Immunoglobulin subtype
IPR003598 565 631 SM00408 Immunoglobulin subtype 2
IPR003599 650 729 SM00409 Immunoglobulin subtype
IPR003598 656 722 SM00408 Immunoglobulin subtype 2
IPR003599 741 820 SM00409 Immunoglobulin subtype
IPR003598 747 813 SM00408 Immunoglobulin subtype 2
IPR003599 832 911 SM00409 Immunoglobulin subtype
IPR003598 838 904 SM00408 Immunoglobulin subtype 2
IPR003599 924 1007 SM00409 Immunoglobulin subtype
IPR003598 930 996 SM00408 Immunoglobulin subtype 2
IPR003599 1020 1099 SM00409 Immunoglobulin subtype
IPR003598 1026 1092 SM00408 Immunoglobulin subtype 2
ProfileScan IPR007110 79 166 PS50835 Immunoglobulin-like
IPR007110 175 261 PS50835 Immunoglobulin-like
IPR003961 353 446 PS50853 Fibronectin
IPR007110 550 636 PS50835 Immunoglobulin-like
IPR007110 640 729 PS50835 Immunoglobulin-like
IPR007110 738 818 PS50835 Immunoglobulin-like
IPR007110 822 911 PS50835 Immunoglobulin-like
IPR007110 913 1005 PS50835 Immunoglobulin-like
IPR007110 1009 1087 PS50835 Immunoglobulin-like
IPR007110 1101 1140 PS50835 Immunoglobulin-like
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f GCTCTTTGTGTACTGGTGTCC
Primer_r GCAAACGCCTTCCAAGCTGTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name GeneBridge 4
Primer_f GCTCTTTGTGTACTGGTGTCC
Primer_r GCAAACGCCTTCCAAGCTGTC
PCR product length 154 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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