Gene/Protein Characteristic Table for KIAA0653
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Product ID ORK05481
Accession No AB014553
Description inducible T-cell co-stimulator ligand
Clone name hk01718
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4358 bp)
Predicted protein sequence (558 aa)
Source Human adult brain
Rouge ID mKIAA0653 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4358 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Features of the protein sequence
Description

Length: 558 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAX09447 4.1e-161 91.3 inducible T-cel...
Homo sapiens
O75144 1.2e-100 100.0 ICOS ligand; B7...
Homo sapiens
AAF34739 3.2e-100 100.0 B7-like protein...
Homo sapiens
XP_531578 1.1e-98 98.3 similar to B7-l...
Pan troglodytes
BAH11654 3.6e-70 100.0 unnamed protein...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR013106 57 173 PF07686 Immunoglobulin V-set
HMMSmart IPR003599 64 177 SM00409 Immunoglobulin subtype
ProfileScan IPR007110 36 171 PS50835 Immunoglobulin-like
IPR007110 183 269 PS50835 Immunoglobulin-like

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 297 ATWSILAVLCLLVVVAVAIGWVC 319 PRIMARY 23
Expression profile
Description

RT-PCR
RT-PCR-ELISA
Experimental conditions
Primer_f AGCTTCTGTCCCTCATGCCTG
Primer_r GGGTAGGTCTCACAAATGCCG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 21
Experimental conditions
Panel name GeneBridge 4
Primer_f AGCTTCTGTCCCTCATGCCTG
Primer_r GGGTAGGTCTCACAAATGCCG
PCR product length 172 bp
PCR conditions 95 °C15 sec66 °C60 sec30 cycles
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