Gene/Protein Characteristic Table for KIAA0475
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Product ID ORK00545
Accession No AB007944
Description family with sequence similarity 20, member B
Clone name hh00451
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5983 bp)
Predicted protein sequence (438 aa)
Flexi ORF Clone FXC00545
Source Human adult brain
Rouge ID mKIAA0475 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5983 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 4417 bp
Genome contig ID gi89161185f_177179470
PolyA signal sequence
(AATAAA,-7)
+----*----+----*----+----*----+----
CTGAAAAAAGAAAATGAATAAAGATTTTAATAAAT
Flanking genome sequence
(132852 - 132901)
----+----*----+----*----+----*----+----*----+----*
ATTGATATCTGATCTTTTCCTCTTCTTTCCCCCTTCAGGATATTTCCAGT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 f 177261654 177312320 8 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 438 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O75063 1.7e-185 100.0 Protein FAM20B;...
Homo sapiens
XP_514027 3.8e-184 99.0 family with seq...
Pan troglodytes
XP_001115532 2.6e-183 98.8 family with seq...
Macaca mulatta
Q8VCS3 5.7e-182 97.3 Protein FAM20B;...
Mus musculus
XP_537175 9.3e-182 97.8 similar to Prot...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR009581 204 431 PF06702 Protein of unknown function DUF1193

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 35 RVVLLAILLVIFIFTKVFLIDNL 57 PRIMARY 23
Experimental conditions
Primer_f
Primer_r
PCR conditions °C sec °C sec cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name GeneBridge 4
Primer_f ATCAAAAGAGAGGGTAGTGGC
Primer_r GGTGAAACTGGGAACAACTAC
PCR product length 81 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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