FAM20B ORF clone FHC00545

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Halotag ORF Clone FHC00545

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Clone info.

Species

human

Clone Name

pFN21ASDA0475 Sequence (with vector) Restriction Map

Product ID

FHC00545

Flexi Clone PID

FXC00545

ORF Sequence

DNA sequence (1227 bp)
Protein sequence (409 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383891]

Gene

KIAA0475

Symbol

FAM20B

Alias : gxk1

Description

family with sequence similarity 20, member B

Original Clone ID

hh00451

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1227 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 409 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1328.1 1.7e-188 100.0 FAM20B CCDS47522.1 6.6e-60 42.5 FAM20C CCDS11679.1 5.6e-51 37.5 FAM20A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam NULL 190 402 PF06702 NULL
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 6 RVVLLAILLVIFIFTKVFLIDNL 28 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055679 409 611063 100.0 100.0 NP_001311239 409 611063 100.0 100.0 NP_001311240 409 611063 100.0 100.0 XP_016858490 432 611063 94.7 100.0 XP_016858491 432 611063 94.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0475 Download

>KIAA0475  1227 bp
ATGAAGCTAAAGCAGCGAGTCGTGCTGTTAGCAATTCTCCTTGTCATTTTTATCTTCACC
AAAGTTTTCCTGATTGACAACTTAGATACATCAGCTGCCAACCGGGAGGACCAGAGGGCC
TTTCACCGAATGATGACTGGCTTGCGGGTGGAGCTGGCACCCAAGCTGGACCATACCTTG
CAGTCTCCCTGGGAGATTGCAGCCCAGTGGGTGGTTCCCCGGGAAGTGTACCCTGAAGAG
ACACCAGAGCTGGGGGCAGTCATGCATGCCATGGCCACCAAGAAAATCATTAAAGCTGAT
GTGGGTTATAAAGGGACACAGCTGAAAGCCTTACTGATACTTGAAGGAGGCCAGAAAGTT
GTTTTCAAACCTAAGCGGTATAGCCGAGACCATGTGGTGGAAGGGGAACCGTATGCTGGT
TATGATAGACACAATGCAGAGGTAGCAGCCTTTCACTTGGACAGGATTCTGGGTTTCCAC
CGAGCCCCCTTGGTAGTTGGCAGATTTGTTAATCTTCGGACAGAGATCAAACCTGTCGCC
ACAGAGCAGCTGTTGAGCACCTTCCTAACTGTAGGAAACAATACTTGTTTTTATGGGAAG
TGCTATTACTGCCGAGAAACAGAACCAGCTTGTGCTGATGGAGACATAATGGAGGGATCT
GTCACACTTTGGCTTCCAGATGTGTGGCCTCTGCAGAAGCACCGTCACCCATGGGGCAGG
ACTTACCGAGAAGGCAAATTGGCCAGGTGGGAGTATGATGAGAGCTACTGTGATGCTGTG
AAGAAAACGTCCCCTTATGACTCTGGCCCGCGCCTCTTGGACATCATTGACACAGCTGTC
TTTGATTACCTGATTGGCAATGCTGACCGCCATCACTATGAGAGCTTTCAAGATGATGAA
GGCGCTAGTATGCTCATCCTTCTTGATAATGCCAAAAGCTTTGGGAACCCCTCGCTGGAT
GAAAGAAGCATTCTTGCCCCTCTCTATCAGTGTTGCATCATTCGGGTGTCCACCTGGAAC
AGACTGAACTACCTAAAGAATGGTGTGCTAAAGTCTGCCTTAAAATCTGCCATGGCCCAT
GACCCCATCTCCCCAGTGCTCTCTGATCCTCATCTGGACGCCGTGGACCAGCGGCTCCTG
AGTGTCCTGGCCACCGTGAAGCAGTGCACCGACCAGTTTGGGATGGACACAGTACTGGTG
GAAGACAGGATGCCTCTCTCACACTTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0475 Download

>KIAA0475  409 aa
MKLKQRVVLLAILLVIFIFTKVFLIDNLDTSAANREDQRAFHRMMTGLRVELAPKLDHTL
QSPWEIAAQWVVPREVYPEETPELGAVMHAMATKKIIKADVGYKGTQLKALLILEGGQKV
VFKPKRYSRDHVVEGEPYAGYDRHNAEVAAFHLDRILGFHRAPLVVGRFVNLRTEIKPVA
TEQLLSTFLTVGNNTCFYGKCYYCRETEPACADGDIMEGSVTLWLPDVWPLQKHRHPWGR
TYREGKLARWEYDESYCDAVKKTSPYDSGPRLLDIIDTAVFDYLIGNADRHHYESFQDDE
GASMLILLDNAKSFGNPSLDERSILAPLYQCCIIRVSTWNRLNYLKNGVLKSALKSAMAH
DPISPVLSDPHLDAVDQRLLSVLATVKQCTDQFGMDTVLVEDRMPLSHL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0475 Download

>pFN21ASDA0475  5956 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGAAGCTAAAGCAGCG
AGTCGTGCTGTTAGCAATTCTCCTTGTCATTTTTATCTTCACCAAAGTTTTCCTGATTGA
CAACTTAGATACATCAGCTGCCAACCGGGAGGACCAGAGGGCCTTTCACCGAATGATGAC
TGGCTTGCGGGTGGAGCTGGCACCCAAGCTGGACCATACCTTGCAGTCTCCCTGGGAGAT
TGCAGCCCAGTGGGTGGTTCCCCGGGAAGTGTACCCTGAAGAGACACCAGAGCTGGGGGC
AGTCATGCATGCCATGGCCACCAAGAAAATCATTAAAGCTGATGTGGGTTATAAAGGGAC
ACAGCTGAAAGCCTTACTGATACTTGAAGGAGGCCAGAAAGTTGTTTTCAAACCTAAGCG
GTATAGCCGAGACCATGTGGTGGAAGGGGAACCGTATGCTGGTTATGATAGACACAATGC
AGAGGTAGCAGCCTTTCACTTGGACAGGATTCTGGGTTTCCACCGAGCCCCCTTGGTAGT
TGGCAGATTTGTTAATCTTCGGACAGAGATCAAACCTGTCGCCACAGAGCAGCTGTTGAG
CACCTTCCTAACTGTAGGAAACAATACTTGTTTTTATGGGAAGTGCTATTACTGCCGAGA
AACAGAACCAGCTTGTGCTGATGGAGACATAATGGAGGGATCTGTCACACTTTGGCTTCC
AGATGTGTGGCCTCTGCAGAAGCACCGTCACCCATGGGGCAGGACTTACCGAGAAGGCAA
ATTGGCCAGGTGGGAGTATGATGAGAGCTACTGTGATGCTGTGAAGAAAACGTCCCCTTA
TGACTCTGGCCCGCGCCTCTTGGACATCATTGACACAGCTGTCTTTGATTACCTGATTGG
CAATGCTGACCGCCATCACTATGAGAGCTTTCAAGATGATGAAGGCGCTAGTATGCTCAT
CCTTCTTGATAATGCCAAAAGCTTTGGGAACCCCTCGCTGGATGAAAGAAGCATTCTTGC
CCCTCTCTATCAGTGTTGCATCATTCGGGTGTCCACCTGGAACAGACTGAACTACCTAAA
GAATGGTGTGCTAAAGTCTGCCTTAAAATCTGCCATGGCCCATGACCCCATCTCCCCAGT
GCTCTCTGATCCTCATCTGGACGCCGTGGACCAGCGGCTCCTGAGTGTCCTGGCCACCGT
GAAGCAGTGCACCGACCAGTTTGGGATGGACACAGTACTGGTGGAAGACAGGATGCCTCT
CTCACACTTGTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT