Gene/Protein Characteristic Table for KIAA0443
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Product ID ORK00078
Accession No AB007903
Description G protein-coupled receptor associated sorting protein 1, transcript variant 1
Clone name pj00552
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4705 bp)
Predicted protein sequence (1404 aa)
Flexi ORF Clone FXC00078
Source Human brain (hippocampus)
Rouge ID mKIAA0443 by Kazusa Mouse cDNA Project
Note We replaced hj00137, former representative clones for KIAA0443 with pj00552. (2004/1/10)
Features of the cloned cDNA sequence
Description

Length: 4705 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 351 bp
Genome contig ID gi89161218f_101695332
PolyA signal sequence
(AATAAA,-25)
+----*----+----*----+----*----+----
TTTGTGTTTCAATAAAGTCCTATGTTAAAGTTGGC
Flanking genome sequence
(104706 - 104755)
----+----*----+----*----+----*----+----*----+----*
AGAAATCACCCTTCTTCTTGAAATTAAAATACAGACCCAATGATAACACA
Features of the protein sequence
Description

Length: 1404 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
BAG11192 0 100.0 G-protein coupl...
synthetic construct
Q5JY77 0 99.9 G-protein coupl...
Homo sapiens
XP_001082557 0 95.1 similar to G pr...
Macaca mulatta
XP_521188 0 58.8 G protein-coupl...
Pan troglodytes
EDL85822 1.4e-191 57.5 rCG20046 [Rattu...
Rattus norvegicus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB051488 6.4e-36 29.5 KIAA1701
AB011084 1.5e-13 28.4 KIAA0512
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR006911 1141 1393 PF04826 Protein of unknown function DUF634
ScanRegExp IPR002345 409 420 PS00213 Lipocalin
IPR002345 783 794 PS00213 Lipocalin
IPR002345 971 984 PS00213 Lipocalin
IPR002345 1005 1017 PS00213 Lipocalin
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f AACGACAATCAGAAGGGTGGC
Primer_r GATGCCAGCTGAATATTAGAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 9
Experimental conditions
Panel name GeneBridge 4
Primer_f AACGACAATCAGAAGGGTGGC
Primer_r GATGCCAGCTGAATATTAGAG
PCR product length 128 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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