##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AviII BbeI BfrI BglI BsaBI
BsaHI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CfoI ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 2725 TCCGGA T^CCGGA
AflIII 3251 ACGTGT A^C[GA][CT]GT
ApaI 986 GGGCCC GGGCC^C
AseI 3649 ATTAAT AT^TAAT
AsnI 3649 ATTAAT AT^TAAT
AspHI 896 GAGCTC G[AT]GC[AT]^C
AvrII 698 CCTAGG C^CTAGG
BamHI 3667 GGATCC G^GATCC
BanI 191 GGTGCC G^G[CT][GA]CC
BbrPI 3250 CACGTG CAC^GTG
BcgI 1175 CGAGAAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3919 CCCGG CC^[GC]GG
BglII 101 AGATCT A^GATCT
BlnI 698 CCTAGG C^CTAGG
BlpI 452 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 452 GCTCAGC GC^T[ACGT]AGC
BsaAI 3250 CACGTG [CT]AC^GT[GA]
BsaWI 2725 TCCGGA [AT]^CCGG[AT]
BseAI 2725 TCCGGA T^CCGGA
BsgI 372 GTGCAG GTGCAG(16/14)
BsiHKAI 896 GAGCTC G[AT]GC[AT]^C
BsmI 4268 GCATTC GAATGC(1/-1)
BspEI 2725 TCCGGA T^CCGGA
BsrFI 2228 GCCGGT [GA]^CCGG[CT]
Bst1107I 2823 GTATAC GTA^TAC
BstZ17I 2823 GTATAC GTA^TAC
CelII 452 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2228 GCCGGT [GA]^CCGG[CT]
DraIII 4565 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3332 CCATGG C^C[GA][CT]GG
MaeII 3251 ACGT A^CGT
MroI 2725 TCCGGA T^CCGGA
NciI 3919 CCCGG CC^[GC]GG
NcoI 3332 CCATGG C^CATGG
NdeI 3874 CATATG CA^TATG
PmaCI 3250 CACGTG CAC^GTG
PmlI 3250 CACGTG CAC^GTG
PshAI 2137 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3649 ATTAAT AT^TAAT
SacI 896 GAGCTC GAGCT^C
SmlI 4406 CTTGAG C^T[CT][GA]AG
XbaI 2993 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 4256 CCGC CCGC(-3/-1)
1745 GCGG
Asp700I 3656 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3711 GAAGTGCTTC
AspI 1789 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2270 GACATTGTC
BalI 849 TGGCCA TGG^CCA
3027 TGGCCA
BbsI 1070 GAAGAC GAAGAC(2/6)
2564 GAAGAC
BciVI 2221 GTATCC GTATCC(6/5)
2942 GTATCC
BclI 1275 TGATCA T^GATCA
3478 TGATCA
BpuAI 1070 GAAGAC GAAGAC(2/6)
2564 GAAGAC
BspHI 3679 TCATGA T^CATGA
4089 TCATGA
BspMI 1008 GCAGGT ACCTGC(4/8)
3243 GCAGGT
BstXI 840 CCATGCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3423 CCAGGAACATGG
EcoNI 568 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1260 CCTTTTCAAGG
FbaI 1275 TGATCA T^GATCA
3478 TGATCA
HincII 1688 GTCAAC GT[CT]^[GA]AC
2051 GTCAAC
HindII 1688 GTCAAC GT[CT]^[GA]AC
2051 GTCAAC
MluNI 849 TGGCCA TGG^CCA
3027 TGGCCA
MscI 849 TGGCCA TGG^CCA
3027 TGGCCA
NheI 1517 GCTAGC G^CTAGC
2572 GCTAGC
PflFI 1789 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2270 GACATTGTC
PflMI 972 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2096 CCATGTTTTGG
RcaI 3679 TCATGA T^CATGA
4089 TCATGA
ScaI 138 AGTACT AGT^ACT
533 AGTACT
SexAI 977 ACCTGGT A^CC[AT]GGT
1385 ACCTGGT
TaqI 3541 TCGA T^CGA
3742 TCGA
Tth111I 1789 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2270 GACATTGTC
TthHB8I 3541 TCGA T^CGA
3742 TCGA
Van91I 972 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2096 CCATGTTTTGG
XcmI 298 CCAGATAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1440 CCAGTCTACAAGTGG
XmnI 3656 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3711 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BanII
BbvCI BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII