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Order Kazusa clone(s) from : |
| Product ID | ORK00073 |
|---|---|
| Accession No | AB007884 |
| Description | Cdc42 guanine nucleotide exchange factor (GEF) 9, transcript variant 1 |
| Clone name | hh01267 |
| Vector information | |
| cDNA sequence | DNA sequence (5413 bp) Predicted protein sequence (567 aa) |
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HaloTag ORF Clone |
FHC00073
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| Flexi ORF Clone | FXC00073 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0424
by Kazusa Mouse cDNA Project
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Length: 5413 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 3061 bp |
|---|---|
| Genome contig ID | gi89161218r_62671573 |
| PolyA signal sequence (AATAAA,-20) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (100000 - 99951) |
----+----*----+----*----+----*----+----*----+----* |
Length: 567 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR001452 | 65 | 113 | PD000066 | Src homology-3 |
| FPrintScan | IPR001452 | 76 | 91 | PR00452 | Src homology-3 |
| IPR001452 | 93 | 102 | PR00452 | Src homology-3 | |
| IPR001452 | 104 | 116 | PR00452 | Src homology-3 | |
| HMMPfam | IPR001452 | 77 | 116 | PF00018 | Src homology-3 |
| IPR000219 | 158 | 337 | PF00621 | DH | |
| IPR001849 | 370 | 476 | PF00169 | Pleckstrin-like | |
| HMMSmart | IPR001452 | 62 | 117 | SM00326 | Src homology-3 |
| IPR000219 | 158 | 337 | SM00325 | DH | |
| IPR001849 | 370 | 478 | SM00233 | Pleckstrin-like | |
| ProfileScan | IPR001452 | 59 | 118 | PS50002 | Src homology-3 |
| IPR000219 | 154 | 338 | PS50010 | DH | |
| IPR001849 | 369 | 476 | PS50003 | Pleckstrin-like |
RT-PCR
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|---|
Experimental conditions| Primer_f | GAGCAGCAAGGGTAATCAAAG |
|---|---|
| Primer_r | GTGTGAAGCCAAATAGTGATC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GAGCAGCAAGGGTAATCAAAG |
| Primer_r | GTGTGAAGCCAAATAGTGATC |
| PCR product length | 113 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |