Gene/Protein Characteristic Table for KIAA0424
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Product ID ORK00073
Accession No AB007884
Description Cdc42 guanine nucleotide exchange factor (GEF) 9, transcript variant 1
Clone name hh01267
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5413 bp)
Predicted protein sequence (567 aa)
Flexi ORF Clone FXC00073
Source Human adult brain
Rouge ID mKIAA0424 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5413 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 3061 bp
Genome contig ID gi89161218r_62671573
PolyA signal sequence
(AATAAA,-20)
+----*----+----*----+----*----+----
ATATTTATGGAGTTCAATAAAAGAAGTAAAAAGAC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACCTCCTGGCATAGCTCTCGTTCGTGTGTGTGTGTGTGTGTGTGTGTGTG
Features of the protein sequence
Description

Length: 567 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O43307 2.6e-212 100.0 Rho guanine nuc...
Homo sapiens
BAF82682 5.4e-212 99.8 unnamed protein...
Homo sapiens
EAX05416 2e-211 99.8 Cdc42 guanine n...
Homo sapiens
XP_001489431 3.4e-211 99.6 similar to Cdc4...
Equus caballus
CAM18135 4.5e-210 98.8 Cdc42 guanine n...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB029035 1.5e-91 58.5 KIAA1112
AB037836 4.6e-22 34.2 KIAA1415
AB002360 1.8e-09 24.3 KIAA0362
AB018336 4.6e-09 24.1 KIAA0793
AB002335 2.8e-08 23.0 KIAA0337
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR001452 65 113 PD000066 Src homology-3
FPrintScan IPR001452 76 91 PR00452 Src homology-3
IPR001452 93 102 PR00452 Src homology-3
IPR001452 104 116 PR00452 Src homology-3
HMMPfam IPR001452 77 116 PF00018 Src homology-3
IPR000219 158 337 PF00621 DH
IPR001849 370 476 PF00169 Pleckstrin-like
HMMSmart IPR001452 62 117 SM00326 Src homology-3
IPR000219 158 337 SM00325 DH
IPR001849 370 478 SM00233 Pleckstrin-like
ProfileScan IPR001452 59 118 PS50002 Src homology-3
IPR000219 154 338 PS50010 DH
IPR001849 369 476 PS50003 Pleckstrin-like
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f GAGCAGCAAGGGTAATCAAAG
Primer_r GTGTGAAGCCAAATAGTGATC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. X
Experimental conditions
Panel name GeneBridge 4
Primer_f GAGCAGCAAGGGTAATCAAAG
Primer_r GTGTGAAGCCAAATAGTGATC
PCR product length 113 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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