##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AflII Alw44I Aor51HI ApaLI AscI
AseI AsnI AviII BamHI BbrPI
BcgI BfrI BsiWI BsmBI Bsp1407I
BsrGI BssHII BssSI BstAPI BstBI
CpoI DraIII Eco47III EcoRI EcoT22I
FseI FspI KspI MfeI MluI
MunI NdeI NotI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 898 GACGTC GACGT^C
Acc65I 2600 GGTACC G^GTACC
AccI 1575 GTATAC GT^[AC][GT]AC
AccIII 1865 TCCGGA T^CCGGA
AclI 764 AACGTT AA^CGTT
AgeI 124 ACCGGT A^CCGGT
AhdI 4125 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3920 CCTCAGG CC^T[ACGT]AGG
Asp700I 4281 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2600 GGTACC G^GTACC
AspEI 4125 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3295 CCTAGG C^CTAGG
BlnI 3295 CCTAGG C^CTAGG
BlpI 1561 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1561 GCTGAGC GC^T[ACGT]AGC
BsaAI 4435 TACGTA [CT]AC^GT[GA]
BsaBI 3516 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1865 TCCGGA T^CCGGA
BsiEI 1797 CGGCCG CG[GA][CT]^CG
BspDI 1504 ATCGAT AT^CGAT
BspEI 1865 TCCGGA T^CCGGA
BsrBI 2184 CCGCTC CCGCTC(-3/-3)
Bst1107I 1575 GTATAC GTA^TAC
BstEII 4924 GGTAACC G^GT[ACGT]ACC
BstPI 4924 GGTAACC G^GT[ACGT]ACC
BstZ17I 1575 GTATAC GTA^TAC
Bsu36I 3920 CCTCAGG CC^T[ACGT]AGG
CelII 1561 GCTGAGC GC^T[ACGT]AGC
ClaI 1504 ATCGAT AT^CGAT
DrdI 1948 GACTTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1797 CGGCCG C^GGCCG
Eam1105I 4125 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1797 CGGCCG C^GGCCG
Eco52I 1797 CGGCCG C^GGCCG
Eco81I 3920 CCTCAGG CC^T[ACGT]AGG
EcoNI 3755 CCTAGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4924 GGTAACC G^GT[ACGT]ACC
EcoRV 1261 GATATC GAT^ATC
KpnI 2600 GGTACC GGTAC^C
MamI 3516 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1865 TCCGGA T^CCGGA
NaeI 491 GCCGGC GCC^GGC
NgoMIV 491 GCCGGC G^CCGGC
NheI 4224 GCTAGC G^CTAGC
NruI 653 TCGCGA TCG^CGA
PinAI 124 ACCGGT A^CCGGT
Psp1406I 764 AACGTT AA^CGTT
PstI 2948 CTGCAG CTGCA^G
PvuII 1613 CAGCTG CAG^CTG
SapI 4093 GAAGAGC GCTCTTC(1/4)
SexAI 2240 ACCTGGT A^CC[AT]GGT
SnaBI 4435 TACGTA TAC^GTA
XcmI 869 CCAACCGGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4281 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 622 CGCG CG^CG
654 CGCG
AlwNI 468 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2236 CAGTACCTG
ApaI 1024 GGGCCC GGGCC^C
3867 GGGCCC
AspI 3553 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5295 GACTCTGTC
BbsI 4155 GAAGAC GAAGAC(2/6)
1816 GTCTTC
BciVI 1545 GTATCC GTATCC(6/5)
4893 GTATCC
BclI 812 TGATCA T^GATCA
3515 TGATCA
BglII 1535 AGATCT A^GATCT
3323 AGATCT
BpuAI 4155 GAAGAC GAAGAC(2/6)
1816 GTCTTC
BsaWI 124 ACCGGT [AT]^CCGG[AT]
1865 TCCGGA
BsgI 1036 GTGCAG GTGCAG(16/14)
1528 GTGCAG
BspHI 1628 TCATGA T^CATGA
4165 TCATGA
BspLU11I 1214 ACATGT A^CATGT
2618 ACATGT
BsrFI 124 ACCGGT [GA]^CCGG[CT]
491 GCCGGC
BstUI 622 CGCG CG^CG
654 CGCG
BstXI 532 CCATGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2021 CCAAGAAGCTGG
Cfr10I 124 ACCGGT [GA]^CCGG[CT]
491 GCCGGC
DraI 886 TTTAAA TTT^AAA
2375 TTTAAA
FbaI 812 TGATCA T^GATCA
3515 TGATCA
HgaI 147 GACGC GACGC(5/10)
1146 GCGTC
HpaI 2331 GTTAAC GTT^AAC
3684 GTTAAC
MspA1I 1613 CAGCTG C[AC]G^C[GT]G
1810 CAGCGG
MvnI 622 CGCG CG^CG
654 CGCG
NcoI 863 CCATGG C^CATGG
4147 CCATGG
PflFI 3553 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5295 GACTCTGTC
RcaI 1628 TCATGA T^CATGA
4165 TCATGA
SmlI 1165 CTCAAG C^T[CT][GA]AG
2468 CTTGAG
SspI 1678 AATATT AAT^ATT
5378 AATATT
Tth111I 3553 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5295 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII