Gene/Protein Characteristic Table for KIAA0358
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Product ID ORK00059
Accession No AB002356
Description MAP-kinase activating death domain, transcript variant 7
Clone name hh00017
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5942 bp)
Predicted protein sequence (1584 aa)
Flexi ORF Clone FXC00059
Source Human adult brain
Rouge ID mKIAA0358 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5942 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Features of the protein sequence
Description

Length: 1584 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW67936 0 100.0 MAP-kinase acti...
Homo sapiens
Q8WXG6 0 99.8 MAP kinase-acti...
Homo sapiens
AAL40265 0 99.5 insulinoma-gluc...
Homo sapiens
XP_001108263 0 98.7 similar to MAP-...
Macaca mulatta
XP_001107325 0 98.7 similar to MAP-...
Macaca mulatta
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB029014 2.4e-06 24.4 KIAA1091
AB051553 3.7e-05 25.3 KIAA1766
AB007945 3.9e-05 24.5 KIAA0476
AB020677 7.4e-05 27.1 KIAA0870
AB033103 0.00029 27.5 KIAA1277
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR005113 11 101 PF03456 uDENN
IPR001194 175 405 PF02141 DENN
IPR005112 488 561 PF03455 dDENN
HMMSmart IPR005113 11 101 SM00800 uDENN
IPR001194 175 405 SM00799 DENN
IPR005112 488 558 SM00801 dDENN
ProfileScan IPR005113 11 107 PS50946 uDENN
IPR001194 175 405 PS50211 DENN
IPR005112 488 561 PS50947 dDENN

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 303 PLELLGVDACLQVLTCILLEHKV 325 PRIMARY 23
2 336 SMSVMAFVAMIYPLEYMFPVIPL 358 PRIMARY 23
3 369 LLLAPTPYIIGVPASFFLYKLDF 391 SECONDARY 23
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f GAATGCTGACTCCTTGCTTGG
Primer_r TCAAGTAACCGAGGACAGAAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name GeneBridge 4
Primer_f GAATGCTGACTCCTTGCTTGG
Primer_r TCAAGTAACCGAGGACAGAAC
PCR product length 100 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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