##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AccIII AclI AflII AscI AviII
BbrPI BciVI BfrI BseAI BsiEI
BspEI BstBI CpoI EagI EclXI
Eco52I EcoT22I FseI FspI KspI
MfeI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 4239 GGTACC G^GTACC
AgeI 5203 ACCGGT A^CCGGT
Aor51HI 4614 AGCGCT AGC^GCT
ApaI 5604 GGGCCC GGGCC^C
AseI 2135 ATTAAT AT^TAAT
AsnI 2135 ATTAAT AT^TAAT
Asp718I 4239 GGTACC G^GTACC
AvrII 4051 CCTAGG C^CTAGG
BcgI 910 GCATGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 804 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5025 AGATCT A^GATCT
BlnI 4051 CCTAGG C^CTAGG
BlpI 724 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 724 GCTCAGC GC^T[ACGT]AGC
BsaAI 4996 TACGTG [CT]AC^GT[GA]
BsaBI 4283 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 4994 CGTACG C^GTACG
BspDI 942 ATCGAT AT^CGAT
BspHI 1531 TCATGA T^CATGA
BspLU11I 2581 ACATGT A^CATGT
BssHII 4768 GCGCGC G^CGCGC
BssSI 5160 CTCGTG CACGAG(-5/-1)
Bst1107I 766 GTATAC GTA^TAC
BstZ17I 766 GTATAC GTA^TAC
CelII 724 GCTCAGC GC^T[ACGT]AGC
ClaI 942 ATCGAT AT^CGAT
Eco47III 4614 AGCGCT AGC^GCT
EcoRV 4547 GATATC GAT^ATC
HincII 470 GTTAAC GT[CT]^[GA]AC
HindII 470 GTTAAC GT[CT]^[GA]AC
HindIII 3585 AAGCTT A^AGCTT
HpaI 470 GTTAAC GTT^AAC
KpnI 4239 GGTACC GGTAC^C
MamI 4283 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1715 ACGCGT A^CGCGT
PaeR7I 831 CTCGAG C^TCGAG
PflMI 86 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 5203 ACCGGT A^CCGGT
PshBI 2135 ATTAAT AT^TAAT
RcaI 1531 TCATGA T^CATGA
SexAI 3853 ACCAGGT A^CC[AT]GGT
SgrAI 2477 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2885 CCCGGG CCC^GGG
SspI 5871 AATATT AAT^ATT
Van91I 86 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 831 CTCGAG C^TCGAG
XmaCI 2885 CCCGGG C^CCGGG
XmaI 2885 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 766 GTATAC GT^[AC][GT]AC
986 GTAGAC
AflIII 1715 ACGCGT A^C[GA][CT]GT
2581 ACATGT
AhdI 1577 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4645 GACCTACTGTC
Alw44I 3032 GTGCAC G^TGCAC
5124 GTGCAC
ApaLI 3032 GTGCAC G^TGCAC
5124 GTGCAC
Asp700I 199 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1563 GAAGGCCTTC
AspEI 1577 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4645 GACCTACTGTC
AspI 2105 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3716 GACTCTGTC
BbvCI 3244 CCTCAGC CCTCAGC(-5/-2)
1991 GCTGAGG
BclI 3709 TGATCA T^GATCA
4438 TGATCA
BsaWI 4263 ACCGGA [AT]^CCGG[AT]
5203 ACCGGT
BsrDI 1208 GCAATG GCAATG(2/0)
3837 GCAATG
BstEII 4867 GGTGACC G^GT[ACGT]ACC
5199 GGTTACC
BstPI 4867 GGTGACC G^GT[ACGT]ACC
5199 GGTTACC
DraI 1468 TTTAAA TTT^AAA
3517 TTTAAA
DrdI 440 GACACTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
989 GACATCGAGGTC
Eam1105I 1577 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4645 GACCTACTGTC
EcoO65I 4867 GGTGACC G^GT[ACGT]ACC
5199 GGTTACC
FbaI 3709 TGATCA T^GATCA
4438 TGATCA
NcoI 186 CCATGG C^CATGG
1662 CCATGG
PflFI 2105 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3716 GACTCTGTC
SacI 589 GAGCTC GAGCT^C
1010 GAGCTC
ScaI 701 AGTACT AGT^ACT
1833 AGTACT
Tth111I 2105 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3716 GACTCTGTC
XmnI 199 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1563 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PshAI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII