Gene/Protein Characteristic Table for KIAA0353
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Product ID ORK00513
Accession No AB002351
Description synemin, intermediate filament protein, transcript variant A
Clone name hg01758y1
Vector information
The cDNA fragment was originally inserted at ApaI-NotI site ...
cDNA sequence DNA sequence (7381 bp)
Predicted protein sequence (1614 aa)
Flexi ORF Clone FXC00513
Source Human adult brain
Rouge ID mKIAA0353 by Kazusa Mouse cDNA Project
Note We replaced hg01758, former representative clones for KIAA0353 with hg01758y1. (2002/12/27)
Features of the cloned cDNA sequence
Description

Length: 7381 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 2525 bp
Genome contig ID gi51511731f_97362771
PolyA signal sequence
(AATAAA,-15)
+----*----+----*----+----*----+----
ATGTCACAAGAATGTGCAAAAATAAAAATCTGAGG
Flanking genome sequence
(130542 - 130591)
----+----*----+----*----+----*----+----*----+----*
AAAAAACCCACATTGTTCCTAAAGAGAATGAATATTTTCAGTAGATTTTG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 15 f 97462771 97493311 4 99.0 Perfect prediction
Features of the protein sequence
Description

Length: 1614 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAI51244 0 100.0 Desmuslin [Homo...
Homo sapiens
O15061 0 99.9 Synemin; Desmuslin.
Homo sapiens
EAX02231 0 99.7 desmuslin, isof...
Homo sapiens
CAC83859 0 98.3 synemin [Homo s...
Homo sapiens
XP_001108469 0 93.7 desmuslin [Maca...
Macaca mulatta
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB020652 5.6e-10 25.1 KIAA0845
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001664 59 370 PF00038 Intermediate filament protein
ScanRegExp IPR001664 357 365 PS00226 Intermediate filament protein
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f TCAAAACCACCAGTAGGAAAC
Primer_r CTGCACACAAGAGAAATCAAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 15
Experimental conditions
Panel name GeneBridge 4
Primer_f TCAAAACCACCAGTAGGAAAC
Primer_r CTGCACACAAGAGAAATCAAC
PCR product length 128 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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