##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII AccIII AclI AocI Aor51HI
AscI BamHI BbeI BclI BglII
BseAI BsiWI BspDI BspEI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI Eco47III Eco81I FbaI FseI
HpaI KasI MluI MroI NarI
NruI NspV PacI Psp1406I RsrII
SalI SfiI SfoI SfuI SgrAI
SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3927 GGTACC G^GTACC
AflII 5429 CTTAAG C^TTAAG
AgeI 1977 ACCGGT A^CCGGT
AhdI 4767 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3927 GGTACC G^GTACC
AspEI 4767 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4372 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 500 TGCGCA TGC^GCA
AvrII 3725 CCTAGG C^CTAGG
BbrPI 2598 CACGTG CAC^GTG
BbvCI 6390 CCTCAGC CCTCAGC(-5/-2)
BfrI 5429 CTTAAG C^TTAAG
BlnI 3725 CCTAGG C^CTAGG
BssSI 1670 CACGAG CACGAG(-5/-1)
BstAPI 1596 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3624 GGTGACC G^GT[ACGT]ACC
BstPI 3624 GGTGACC G^GT[ACGT]ACC
DrdI 2151 GACACAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4767 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3624 GGTGACC G^GT[ACGT]ACC
EcoRV 6037 GATATC GAT^ATC
EcoT22I 648 ATGCAT ATGCA^T
FspI 500 TGCGCA TGC^GCA
HindIII 5939 AAGCTT A^AGCTT
KpnI 3927 GGTACC GGTAC^C
MfeI 1555 CAATTG C^AATTG
MunI 1555 CAATTG C^AATTG
NdeI 3580 CATATG CA^TATG
NotI 475 GCGGCCGC GC^GGCCGC
NsiI 648 ATGCAT ATGCA^T
PaeR7I 546 CTCGAG C^TCGAG
PflFI 4372 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1977 ACCGGT A^CCGGT
PmaCI 2598 CACGTG CAC^GTG
PmeI 6626 GTTTAAAC GTTT^AAAC
PmlI 2598 CACGTG CAC^GTG
PvuI 3191 CGATCG CGAT^CG
SapI 1918 GAAGAGC GCTCTTC(1/4)
SbfI 1049 CCTGCAGG CCTGCA^GG
SexAI 5146 ACCTGGT A^CC[AT]GGT
SpeI 6658 ACTAGT A^CTAGT
SphI 6167 GCATGC GCATG^C
Sse8387I 1049 CCTGCAGG CCTGCA^GG
SspI 6922 AATATT AAT^ATT
Tth111I 4372 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 546 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 486 GACGCC G[GA]^CG[CT]C
1949 GACGCC
AflIII 232 ACGTGT A^C[GA][CT]GT
6195 ACACGT
AseI 5186 ATTAAT AT^TAAT
6919 ATTAAT
AsnI 5186 ATTAAT AT^TAAT
6919 ATTAAT
BalI 1012 TGGCCA TGG^CCA
5648 TGGCCA
BsaHI 486 GACGCC G[GA]^CG[CT]C
1949 GACGCC
BsaI 93 GAGACC GGTCTC(1/5)
4693 GAGACC
BsaWI 1977 ACCGGT [AT]^CCGG[AT]
3651 ACCGGA
BspHI 6883 TCATGA T^CATGA
7026 TCATGA
EagI 290 CGGCCG C^GGCCG
476 CGGCCG
EclXI 290 CGGCCG C^GGCCG
476 CGGCCG
Eco52I 290 CGGCCG C^GGCCG
476 CGGCCG
EcoRI 1164 GAATTC G^AATTC
2952 GAATTC
Hin1I 486 GACGCC G[GA]^CG[CT]C
1949 GACGCC
HincII 2388 GTCAAC GT[CT]^[GA]AC
3165 GTCAAC
HindII 2388 GTCAAC GT[CT]^[GA]AC
3165 GTCAAC
KspI 473 CCGCGG CCGC^GG
6113 CCGCGG
MluNI 1012 TGGCCA TGG^CCA
5648 TGGCCA
MscI 1012 TGGCCA TGG^CCA
5648 TGGCCA
NaeI 123 GCCGGC GCC^GGC
292 GCCGGC
NcoI 1015 CCATGG C^CATGG
4136 CCATGG
NgoMIV 123 GCCGGC G^CCGGC
292 GCCGGC
PshBI 5186 ATTAAT AT^TAAT
6919 ATTAAT
RcaI 6883 TCATGA T^CATGA
7026 TCATGA
SacII 473 CCGCGG CCGC^GG
6113 CCGCGG
ScaI 4979 AGTACT AGT^ACT
7222 AGTACT
SmaI 2673 CCCGGG CCC^GGG
3367 CCCGGG
XbaI 1898 TCTAGA T^CTAGA
4814 TCTAGA
XmaCI 2673 CCCGGG C^CCGGG
3367 CCCGGG
XmaI 2673 CCCGGG C^CCGGG
3367 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcgI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI