Gene/Protein Characteristic Table for KIAA0145
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Product ID ORK04764
Accession No D63479
Description diacylglycerol kinase, delta 130kDa
Clone name hg01767
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6238 bp)
Predicted protein sequence (1198 aa)
Source Human adult brain
Rouge ID mKIAA0145 by Kazusa Mouse cDNA Project
Note We replaced ha00914, former representative clones for KIAA0145 with hg01767. (2005/08/06)
Features of the cloned cDNA sequence
Description

Length: 6238 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2640 bp
Genome contig ID gi89161199f_233827952
PolyA signal sequence
(AATAAA,-28)
+----*----+----*----+----*----+----
GTGTATAAATAAAACAAGCCTGTTTTTGATCTTCC
Flanking genome sequence
(217532 - 217581)
----+----*----+----*----+----*----+----*----+----*
ATCGCCAGAGTCTTTGTCATCATTCCTTGTGCTTTGACATAAAGTGTTGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 f 233927951 234045482 30 99.5 Perfect prediction
Features of the protein sequence
Description

Length: 1198 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q16760 0 100.0 Diacylglycerol ...
Homo sapiens
BAC11809 0 99.9 diacylglycerol ...
Homo sapiens
EAW71048 0 99.9 diacylglycerol ...
Homo sapiens
BAA11134 0 99.2 diacylglycerol ...
Homo sapiens
XP_001114920 0 97.5 similar to diac...
Macaca mulatta
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB018261 3.6e-16 36.7 KIAA0718
AB028945 0.00011 24.0 KIAA1022
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
BlastProDom IPR001206 306 401 PD005043 Diacylglycerol kinase
IPR000756 879 925 PD002939 Diacylglycerol kinase accessory region
FPrintScan IPR002219 161 170 PR00008 Protein kinase C
IPR002219 174 185 PR00008 Protein kinase C
IPR002219 186 198 PR00008 Protein kinase C
HMMPfam IPR001849 38 130 PF00169 Pleckstrin-like
IPR002219 148 200 PF00130 Protein kinase C
IPR002219 220 273 PF00130 Protein kinase C
IPR001206 305 430 PF00781 Diacylglycerol kinase
IPR000756 747 904 PF00609 Diacylglycerol kinase accessory region
IPR011510 1126 1192 PF07647 Sterile alpha motif homology 2
HMMSmart IPR001849 38 132 SM00233 Pleckstrin-like
IPR002219 148 197 SM00109 Protein kinase C
IPR002219 220 270 SM00109 Protein kinase C
IPR001206 305 430 SM00046 Diacylglycerol kinase
IPR000756 747 904 SM00045 Diacylglycerol kinase accessory region
IPR001660 1126 1192 SM00454 Sterile alpha motif SAM
ProfileScan IPR001849 37 130 PS50003 Pleckstrin-like
IPR002219 147 197 PS50081 Protein kinase C
IPR002219 219 270 PS50081 Protein kinase C
IPR001660 1129 1192 PS50105 Sterile alpha motif SAM
ScanRegExp IPR002219 148 197 PS00479 Protein kinase C
IPR002219 220 270 PS00479 Protein kinase C
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name Genebridge 4
Primer_f TGAGGAAGGAATGAACATGAG
Primer_r AGCACTCAGGTATGGACTATG
PCR product length 313 bp
PCR conditions 95 °C15 sec60 °C120 sec30 cycles
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