FLJ00153

Product ID	ORK06642
Accession No	AK074082
Clone name	sh06127
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5761 bp) Predicted protein sequence (497 aa)
Source	Human spleen
Rouge ID	mFLJ00153 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Length: 497 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84908	2e-146	100.0	FLJ00153 protei...	Homo sapiens
EAW84209	1.5e-135	99.8	ral guanine nuc...	Homo sapiens
Q3MIN7	9.3e-134	99.6	Ral guanine nuc...	Homo sapiens
AAI43531	9.8e-133	98.3	Unknown (protei...	Homo sapiens
EAW84211	3.8e-130	98.3	ral guanine nuc...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037729	9e-21	44.0	KIAA1308
AK090450	2.5e-20	42.4	FLJ00371
AK074114	3e-20	42.4	FLJ00185
AB023176	6.9e-11	41.8	KIAA0959
AB002349	0.001	29.4	KIAA0351

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001895	41	251	PF00617	Guanine-nucleotide dissociation stimulator CDC25
HMMPfam	IPR000159	410	495	PF00788	RA domain
HMMSmart	IPR001895	40	303	SM00147	Guanine-nucleotide dissociation stimulator CDC25
ProfileScan	IPR001895	47	301	PS50009	Guanine-nucleotide dissociation stimulator CDC25
	NULL	331	359	PS50324	NULL
	IPR000694	334	404	PS50099	Proline-rich region
	IPR000159	410	474	PS50200	RA domain
ScanRegExp	IPR001895	218	248	PS00720	Guanine-nucleotide dissociation stimulator CDC25

Primer_f
Primer_r
PCR conditions	test