##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII BbrPI BfrI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eam1105I EcoRI EcoT22I FseI
FspI HincII HindII HpaI KpnI
KspI MfeI MluI MroI MunI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 3439 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 379 GAAGAC GAAGAC(2/6)
BciVI 4552 GGATAC GTATCC(6/5)
BclI 5001 TGATCA T^GATCA
BpuAI 379 GAAGAC GAAGAC(2/6)
BsaAI 3938 CACGTA [CT]AC^GT[GA]
BsaWI 294 ACCGGA [AT]^CCGG[AT]
BsmI 5321 GAATGC GAATGC(1/-1)
BstAPI 1935 GCACAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1646 GGTCACC G^GT[ACGT]ACC
BstPI 1646 GGTCACC G^GT[ACGT]ACC
DraI 5148 TTTAAA TTT^AAA
EcoO65I 1646 GGTCACC G^GT[ACGT]ACC
EcoRV 3852 GATATC GAT^ATC
FbaI 5001 TGATCA T^GATCA
NaeI 4013 GCCGGC GCC^GGC
NdeI 5193 CATATG CA^TATG
NgoMIV 4013 GCCGGC G^CCGGC
PaeR7I 3136 CTCGAG C^TCGAG
PshAI 5640 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2588 CCTGCAGG CCTGCA^GG
SexAI 1274 ACCTGGT A^CC[AT]GGT
Sse8387I 2588 CCTGCAGG CCTGCA^GG
XcmI 2201 CCAAGCGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3136 CTCGAG C^TCGAG
XmnI 3439 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 3979 GACGTC GACGT^C
4004 GACGTC
AflIII 2146 ACGTGT A^C[GA][CT]GT
3795 ACACGT
AspI 2659 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2698 GACTGGGTC
BalI 285 TGGCCA TGG^CCA
4641 TGGCCA
BcgI 3221 CGAGAAACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3801 CGAGCTTCGTGC
BglII 394 AGATCT A^GATCT
4299 AGATCT
BsaBI 966 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4553 GATACCTATC
BsiEI 826 CGGCCG CG[GA][CT]^CG
4011 CGGCCG
Bsp1407I 72 TGTACA T^GTACA
5679 TGTACA
BsrGI 72 TGTACA T^GTACA
5679 TGTACA
BssSI 3753 CTCGTG CACGAG(-5/-1)
4232 CTCGTG
BstXI 4384 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4962 CCAGCTATGTGG
EagI 826 CGGCCG C^GGCCG
4011 CGGCCG
EclXI 826 CGGCCG C^GGCCG
4011 CGGCCG
Eco52I 826 CGGCCG C^GGCCG
4011 CGGCCG
HindIII 563 AAGCTT A^AGCTT
1567 AAGCTT
MamI 966 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4553 GATACCTATC
MluNI 285 TGGCCA TGG^CCA
4641 TGGCCA
MscI 285 TGGCCA TGG^CCA
4641 TGGCCA
PflFI 2659 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2698 GACTGGGTC
PflMI 708 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4613 CCAGGGCCTGG
SapI 3439 GAAGAGC GCTCTTC(1/4)
768 GCTCTTC
SphI 1437 GCATGC GCATG^C
4543 GCATGC
SspBI 72 TGTACA T^GTACA
5679 TGTACA
Tsp509I 3240 AATT ^AATT
5749 AATT
Tth111I 2659 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2698 GACTGGGTC
Van91I 708 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4613 CCAGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII AvrII BamHI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI