DCAF12 ORF clone pFN21ASDA1892

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Halotag ORF Clone pFN21ASDA1892

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Clone info.

Species

human

Clone Name

pFN21ASDA1892 Sequence (with vector) Restriction Map

Product ID

FHC00942

Flexi Clone PID

FXC00942

ORF Sequence

DNA sequence (1359 bp)
Protein sequence (453 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384317]

Gene

KIAA1892

Symbol

DCAF12

Alias : CT102, KIAA1892, TCC52, WDR40A

Description

DDB1 and CUL4 associated factor 12

Original Clone ID

fk04164

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1359 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 453 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6549.1 5.3e-194 100.0 DCAF12 CCDS43991.1 2.3e-119 64.9 DCAF12L2 CCDS14610.1 6.6e-113 61.3 DCAF12L1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019781 181 211 PF00400 WD40 repeat HMMSmart IPR001680 77 116 SM00320 WD40 repeat IPR001680 172 211 SM00320 WD40 repeat IPR001680 236 280 SM00320 WD40 repeat IPR001680 328 366 SM00320 WD40 repeat ProfileScan IPR019782 180 220 PS50082 WD40 repeat 2 IPR017986 180 220 PS50294 WD40-repeat-containing domain Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1892 Download

>KIAA1892  1359 bp
ATGGCCCGGAAAGTAGTTAGCAGGAAGCGGAAAGCGCCCGCCTCGCCGGGAGCTGGGAGC
GACGCTCAGGGCCCGCAGTTTGGCTGGGATCACTCGCTTCACAAAAGGAAAAGACTTCCT
CCTGTGAAGAGATCCTTAGTATACTACTTGAAGAACCGGGAAGTCAGGCTACAGAATGAA
ACCAGCTACTCTCGAGTGTTGCATGGTTATGCAGCACAGCAACTTCCCAGTCTCCTGAAG
GAGAGAGAGTTTCACCTTGGGACCCTTAATAAAGTGTTTGCATCTCAGTGGTTGAATCAT
AGGCAAGTGGTGTGTGGCACAAAATGCAACACGCTATTTGTCGTAGATGTCCAGACAAGC
CAGATCACCAAGATCCCCATTCTGAAAGACCGGGAGCCTGGAGGTGTGACCCAGCAGGGC
TGTGGTATCCATGCCATCGAGCTGAATCCTTCTAGAACACTGCTAGCCACTGGAGGAGAC
AACCCCAACAGTCTTGCCATCTATCGACTACCTACGCTGGATCCTGTGTGTGTAGGAGAT
GATGGACACAAGGACTGGATCTTTTCCATCGCATGGATCAGCGACACTATGGCAGTGTCT
GGCTCACGTGATGGTTCTATGGGACTCTGGGAGGTGACAGATGATGTTTTGACCAAAAGT
GATGCGAGACACAATGTGTCACGGGTCCCTGTGTATGCACACATCACTCACAAGGCCTTA
AAGGACATCCCCAAAGAAGACACAAACCCTGACAACTGCAAGGTTCGGGCTCTGGCCTTC
AACAACAAGAACAAGGAACTGGGAGCAGTGTCTCTGGATGGCTACTTTCATCTCTGGAAG
GCTGAAAATACACTATCTAAGCTCCTCTCCACCAAACTGCCATATTGCCGTGAGAATGTG
TGTCTGGCTTATGGTAGTGAATGGTCAGTTTATGCAGTGGGCTCCCAAGCTCATGTCTCC
TTCTTGGATCCACGGCAGCCATCATACAACGTCAAGTCTGTCTGTTCCAGGGAGCGAGGC
AGTGGAATCCGGTCAGTGAGTTTCTACGAGCACATCATCACTGTGGGAACAGGGCAGGGC
TCCCTGCTGTTCTATGACATCCGAGCTCAGAGATTTCTGGAAGAGAGGCTCTCAGCTTGT
TATGGGTCCAAGCCCAGACTAGCAGGGGAGAATCTGAAACTAACCACTGGCAAAGGCTGG
CTGAATCATGATGAAACCTGGAGGAATTACTTTTCAGACATTGACTTCTTCCCCAATGCT
GTTTACACCCACTGCTACGACTCGTCTGGAACGAAACTCTTTGTGGCAGGAGGTCCCCTC
CCTTCAGGGCTCCATGGAAACTATGCTGGGCTCTGGAGT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1892 Download

>KIAA1892  453 aa
MARKVVSRKRKAPASPGAGSDAQGPQFGWDHSLHKRKRLPPVKRSLVYYLKNREVRLQNE
TSYSRVLHGYAAQQLPSLLKEREFHLGTLNKVFASQWLNHRQVVCGTKCNTLFVVDVQTS
QITKIPILKDREPGGVTQQGCGIHAIELNPSRTLLATGGDNPNSLAIYRLPTLDPVCVGD
DGHKDWIFSIAWISDTMAVSGSRDGSMGLWEVTDDVLTKSDARHNVSRVPVYAHITHKAL
KDIPKEDTNPDNCKVRALAFNNKNKELGAVSLDGYFHLWKAENTLSKLLSTKLPYCRENV
CLAYGSEWSVYAVGSQAHVSFLDPRQPSYNVKSVCSRERGSGIRSVSFYEHIITVGTGQG
SLLFYDIRAQRFLEERLSACYGSKPRLAGENLKLTTGKGWLNHDETWRNYFSDIDFFPNA
VYTHCYDSSGTKLFVAGGPLPSGLHGNYAGLWS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1892 Download

>pFN21ASDA1892  6088 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCCCGGAAAGTAGT
TAGCAGGAAGCGGAAAGCGCCCGCCTCGCCGGGAGCTGGGAGCGACGCTCAGGGCCCGCA
GTTTGGCTGGGATCACTCGCTTCACAAAAGGAAAAGACTTCCTCCTGTGAAGAGATCCTT
AGTATACTACTTGAAGAACCGGGAAGTCAGGCTACAGAATGAAACCAGCTACTCTCGAGT
GTTGCATGGTTATGCAGCACAGCAACTTCCCAGTCTCCTGAAGGAGAGAGAGTTTCACCT
TGGGACCCTTAATAAAGTGTTTGCATCTCAGTGGTTGAATCATAGGCAAGTGGTGTGTGG
CACAAAATGCAACACGCTATTTGTCGTAGATGTCCAGACAAGCCAGATCACCAAGATCCC
CATTCTGAAAGACCGGGAGCCTGGAGGTGTGACCCAGCAGGGCTGTGGTATCCATGCCAT
CGAGCTGAATCCTTCTAGAACACTGCTAGCCACTGGAGGAGACAACCCCAACAGTCTTGC
CATCTATCGACTACCTACGCTGGATCCTGTGTGTGTAGGAGATGATGGACACAAGGACTG
GATCTTTTCCATCGCATGGATCAGCGACACTATGGCAGTGTCTGGCTCACGTGATGGTTC
TATGGGACTCTGGGAGGTGACAGATGATGTTTTGACCAAAAGTGATGCGAGACACAATGT
GTCACGGGTCCCTGTGTATGCACACATCACTCACAAGGCCTTAAAGGACATCCCCAAAGA
AGACACAAACCCTGACAACTGCAAGGTTCGGGCTCTGGCCTTCAACAACAAGAACAAGGA
ACTGGGAGCAGTGTCTCTGGATGGCTACTTTCATCTCTGGAAGGCTGAAAATACACTATC
TAAGCTCCTCTCCACCAAACTGCCATATTGCCGTGAGAATGTGTGTCTGGCTTATGGTAG
TGAATGGTCAGTTTATGCAGTGGGCTCCCAAGCTCATGTCTCCTTCTTGGATCCACGGCA
GCCATCATACAACGTCAAGTCTGTCTGTTCCAGGGAGCGAGGCAGTGGAATCCGGTCAGT
GAGTTTCTACGAGCACATCATCACTGTGGGAACAGGGCAGGGCTCCCTGCTGTTCTATGA
CATCCGAGCTCAGAGATTTCTGGAAGAGAGGCTCTCAGCTTGTTATGGGTCCAAGCCCAG
ACTAGCAGGGGAGAATCTGAAACTAACCACTGGCAAAGGCTGGCTGAATCATGATGAAAC
CTGGAGGAATTACTTTTCAGACATTGACTTCTTCCCCAATGCTGTTTACACCCACTGCTA
CGACTCGTCTGGAACGAAACTCTTTGTGGCAGGAGGTCCCCTCCCTTCAGGGCTCCATGG
AAACTATGCTGGGCTCTGGAGTTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT