(Circular) (Six-base) MAPSORT of: pFN21ASDA1892.seq Check: 8264 from: 1 to: 6088 pFN21ASDA1892 6088 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2163 3431 1937 Size: 226 1268 4594 Fragments arranged by size: 4594 1268 226 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 3330 4151 5535 5803 1632 Size: 647 1051 821 1384 268 1917 Fragments arranged by size: 1917 1384 1051 821 647 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2247 1184 Size: 1063 5025 AlwNI CAG_nnn'CTG Cuts at: 2621 3831 5403 2621 Size: 1210 1572 3306 Fragments arranged by size: 3306 1572 1210 ApaI G_GGCC'C Cuts at: 2096 2096 Size: 6088 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 3397 3623 3775 5884 1493 Size: 1904 226 152 2109 1697 Fragments arranged by size: 2109 1904 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2214 3413 1870 Size: 73 271 1199 4545 Fragments arranged by size: 4545 1199 271 73 BamHI G'GATC_C Cuts at: 2542 2989 3418 3785 2542 Size: 447 429 367 4845 Fragments arranged by size: 4845 447 429 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 4864 618 Size: 325 217 627 1622 1455 1842 Fragments arranged by size: 1842 1622 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2096 2794 2966 3104 3130 3354 3375 3407 Size: 705 698 172 138 26 224 21 32 Cuts at: 3407 1391 Size: 4072 Fragments arranged by size: 4072 705 698 224 172 138 32 26 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2766 928 Size: 971 867 4250 Fragments arranged by size: 4250 971 867 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2459 3980 5190 2459 Size: 1521 1210 3357 Fragments arranged by size: 3357 1521 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2096 4149 5305 1790 Size: 306 2053 1156 2573 Fragments arranged by size: 2573 2053 1156 306 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2244 2831 3819 Size: 416 53 83 399 24 827 587 988 Cuts at: 3819 4777 442 Size: 958 1753 Fragments arranged by size: 1753 988 958 827 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2489 1056 Size: 1433 4655 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2103 3169 3356 2103 Size: 1066 187 4835 Fragments arranged by size: 4835 1066 187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 2514 3262 3397 4748 1239 Size: 1203 72 748 135 1351 2579 Fragments arranged by size: 2579 1351 1203 748 135 72 Bpu10I CC'TnA_GC Cuts at: 2088 2088 Size: 6088 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2630 3385 3879 493 Size: 711 1426 755 494 2702 Fragments arranged by size: 2702 1426 755 711 494 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3051 4521 4919 5193 5340 5751 3051 Size: 1470 398 274 147 411 3388 Fragments arranged by size: 3388 1470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2255 2285 1178 Size: 30 165 30 852 30 4981 Fragments arranged by size: 4981 852 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2511 2876 1186 Size: 1325 365 4398 Fragments arranged by size: 4398 1325 365 BseYI C'CCAG_C Cuts at: 2075 2106 2433 3368 5291 5787 2075 Size: 31 327 935 1923 496 2376 Fragments arranged by size: 2376 1923 935 496 327 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3075 3130 4149 4234 5305 3075 Size: 55 1019 85 1071 3858 Fragments arranged by size: 3858 1071 1019 85 55 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2794 2966 3075 3104 3130 3354 Size: 399 306 698 172 109 29 26 224 Cuts at: 3354 3375 3407 4149 4234 5305 1391 Size: 21 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 698 399 306 224 172 109 85 32 29 26 21 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3229 3977 5707 3229 Size: 748 1730 3610 Fragments arranged by size: 3610 1730 748 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4732 4919 1700 Size: 110 2922 187 2869 Fragments arranged by size: 2922 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 2346 794 Size: 1552 4536 BstBI TT'CG_AA Cuts at: 2007 4892 2007 Size: 2885 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2153 2394 2542 2580 2989 3418 3785 4170 Size: 181 241 148 38 409 429 367 385 Cuts at: 4170 4187 5639 5725 5737 6083 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 429 409 385 367 346 241 181 148 86 38 17 12 BstZ17I GTA'TAC Cuts at: 2164 2164 Size: 6088 BtgI C'CryG_G Cuts at: 513 1065 2022 2993 3355 513 Size: 552 957 971 362 3246 Fragments arranged by size: 3246 971 957 552 362 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2575 487 Size: 683 56 1349 4000 Fragments arranged by size: 4000 1349 683 56 BtsI GCAGTG_nn' Cuts at: 2480 2622 2835 2964 3049 3292 3604 4398 4418 Size: 142 213 129 85 243 312 794 20 Cuts at: 4418 2480 Size: 4150 Fragments arranged by size: 4150 794 312 243 213 142 129 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DraIII CAC_nnn'GTG Cuts at: 2698 2698 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053 Size: 54 1425 50 275 18 1705 889 1629 Cuts at: 6053 8 Size: 43 Fragments arranged by size: 1705 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 3138 4018 1353 Size: 791 994 880 3423 Fragments arranged by size: 3423 994 880 791 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3128 3128 Size: 6088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2442 2514 3262 3330 3397 4151 Size: 393 647 163 72 748 68 67 754 Cuts at: 4151 4748 5535 5803 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 754 748 647 597 393 268 163 72 68 67 EcoO109I rG'GnC_Cy Cuts at: 2092 2283 2707 3335 2092 Size: 191 424 628 4845 Fragments arranged by size: 4845 628 424 191 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2060 5235 1678 Size: 382 3175 2531 Fragments arranged by size: 3175 2531 382 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4729 4761 4803 4835 1373 Size: 32 135 32 3157 32 42 32 2626 Fragments arranged by size: 3157 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2229 2308 3083 4046 4405 4564 518 Size: 648 1063 79 775 963 359 159 2042 Fragments arranged by size: 2042 1063 963 775 648 359 159 79 MspA1I CmG'CkG Cuts at: 1606 3504 4181 5329 5574 1606 Size: 1898 677 1148 245 2120 Fragments arranged by size: 2120 1898 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 3355 513 Size: 552 957 1333 3246 Fragments arranged by size: 3246 1333 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 2485 1052 Size: 1433 4655 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 3446 4991 1132 Size: 97 2217 1545 2229 Fragments arranged by size: 2229 2217 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2595 1108 Size: 1487 4601 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PmlI CAC'GTG Cuts at: 2630 2630 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2283 2707 3335 2283 Size: 424 628 5036 Fragments arranged by size: 5036 628 424 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2092 2092 Size: 6088 PstI C_TGCA'G Cuts at: 830 3440 830 Size: 2610 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 SacI G_AGCT'C Cuts at: 3130 3130 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SanDI GG'GwC_CC Cuts at: 2283 2707 2283 Size: 424 5664 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2192 3436 4571 5252 5443 826 Size: 219 1147 1244 1135 681 191 1471 Fragments arranged by size: 1471 1244 1147 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2214 4192 5093 5355 820 Size: 197 926 271 1978 901 262 1553 Fragments arranged by size: 1978 1553 926 901 271 262 197 SnaBI TAC'GTA Cuts at: 493 3385 3879 493 Size: 2892 494 2702 Fragments arranged by size: 2892 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 2738 1517 Size: 1221 4867 StyI C'CwwG_G Cuts at: 513 1065 2022 2278 3355 3527 513 Size: 552 957 256 1077 172 3074 Fragments arranged by size: 3074 1077 957 552 256 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3305 4115 4132 4285 4470 4915 325 Size: 1255 1725 810 17 153 185 445 1498 Fragments arranged by size: 1725 1498 1255 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 6048 6080 2534 Size: 32 3482 32 2542 Fragments arranged by size: 3482 2542 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 2474 3424 2474 Size: 950 5138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2441 2441 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 2214 1943 Size: 271 5817 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI